
Citation: LI Yingying, WANG Dingyi, NONG Qiying, LIU Lihong, ZHANG Meng, LIANG Yong, HU Ligang, HE Bin, JIANG Guibin. 3D printed portable gel electrophoresis device for rapid detection of proteins[J]. Chinese Journal of Chromatography, 2020, 38(11): 1316-1322. doi: 10.3724/SP.J.1123.2020.02018

3D打印便携式凝胶电泳装置用于蛋白质的快速检测
English
3D printed portable gel electrophoresis device for rapid detection of proteins
In summary, this research designed and manufactured a portable gel electrophoresis device using 3D printing technique, which can be used for on-site analysis and detection of proteins. The device presents the following advantages compared with the commercial devices:1) small and portable:the size of the electrophoresis tank of the device is only 15 mm×20 mm×17 mm and the 25 V lithium battery has a working time of approximately 100 hours; 2) low cost:it can be processed in 5 hours using 3D printing technology, with 10 mL of printing material while the total cost is less than 400 RMB; 3) fast separation:this device can quickly achieve protein separation compared with commercial devices and can further use multiple electrophoresis tanks in parallel to analyze more samples at the same time. Besides, this research also highlights the advantages of 3D printing for the development of miniaturized analytical equipment. Though this study has achieved preliminary results for rapid separation of proteins using gel electrophoresis devices, the quantitative analysis of proteins following protein detection and the application of more samples need further research. Meanwhile, the continued application of 3D printing technology will promote the development of miniaturized and portable experimental equipment.
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Key words:
- gel electrophoresis
- / 3D printing
- / protein
- / portable
- / separation
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[1] Pan J Z, Fang P, Fang X X, et al. Sci Rep, 2018, 8(1):1791
-
[2] Guan Y F, Wang J W, Duan C F. Chinese Journal of Chromatography, 2009, 27(5):592 关亚风, 王建伟, 段春凤. 色谱, 2009, 27(5):592
-
[3] Badr I H A, Johnson R D, Madou M J, et al. Anal Chem, 2002, 74(21):5569
-
[4] Figeys D, Pinto D. Anal Chem, 2000, 72(9):330A
-
[5] Nachamkin I, Panaro N J, Li M, et al. J Clin Microbiol, 2001, 39(2):754
-
[6] Renzi R F, Stamps J, Horn B A, et al. Anal Chem, 2005, 77(2):435
-
[7] Skelley A M, Scherer J R, Aubrey A D, et al. Proc Natl Acad Sci U S A, 2005, 102(4):1041
-
[8] Liu R H, Lodes M J, Nguyen T, et al. Anal Chem, 2006, 78(12):4184
-
[9] Sista R, Hua Z, Thwar P, et al. Lab Chip, 2008, 8(12):2091
-
[10] Yuan Y X, Fan C, Pan J Z, et al. Chinese Journal of Chromatography, 2020, 38(2):183 袁颖欣, 樊晨, 潘建章, 等. 色谱, 2020, 38(2):183
-
[11] Lee B S, Lee Y U, Kim H S, et al. Lab Chip, 2011, 11(1):70
-
[12] Yin H F, Killeen K. J Sep Sci, 2007, 30(10):1427
-
[13] Li Y, Dvorak M, Nesterenko P N, et al. Anal Chim Acta, 2015, 896:166
-
[14] Cong W T, Zhou A, Liu Z G, et al. Anal Chem, 2015, 87(3):1462
-
[15] Hu L, Cheng T, He B, et al. Angew Chem Int Ed Engl, 2013, 52(18):4916
-
[16] Yan X T, He B, Wang D Y, et al. Talanta, 2018, 184:404
-
[17] Zhu G L, Yang Z H. Plant Physiology Communications, 1982(2):43 朱广廉, 杨中汉. 植物生理学通讯, 1982(2):43
-
[18] Shi J H, Zhao Y T, Wang J L, et al. Journal of the Fourth Military Medical University, 2000, 21(6):761 石继红, 赵永同, 王俊楼, 等. 第四军医大学学报, 2000, 21(6):761
-
[19] Zhao X, Yang L, Yu Z, et al. J Environ Sci, 2008, 20(2):224
-
[20] Wang D Y, He B, Yan X T, et al. Talanta, 2019, 197:145
-
[21] Gross B, Lockwood S Y, Spence D M. Anal Chem, 2017, 89(1):57
-
[22] Dixit C K, Kadimisetty K, Rusling J. TrAC-Trends Anal Chem, 2018, 106:37
-
[23] Gaal G, Mendes M, De Almeida T P, et al. Sens Actuator B-Chem, 2017, 242:35
-
[24] Su C K, Hsieh M H, Sun Y C. Anal Chim Acta, 2016, 914:110
-
[25] Lücking T H, Sambale F, Beutel S, et al. Eng Life Sci, 2015, 15(1):51
-
[26] Simon U, Dimartino S. J Chromatogr A, 2019, 1587:119
-
[27] Calderilla C, Maya F, Cerda V, et al. Talanta, 2019, 202:67
-
[28] Macdonald N P, Currivan S A, Tedone L, et al. Anal Chem, 2017, 89(4):2457
-
[29] Zhang C, Glaros T, Manicke N E. J Am Chem Soc, 2017, 139(32):10996
-
[30] Dou M, Sanchez J, Tavakoli H, et al. Anal Chim Acta, 2019, 1065:71
-
[31] Rong Z, Wang Q, Sun N X, et al. Anal Chim Acta, 2019, 1055:140
-
[32] Hu L G, Jiang G B. Environ Sci Technol, 2017, 51(7):3597
-
[33] Wang D, Huang X, Li J, et al. Chem Commun, 2018, 54(22):2723
-
[34] Au A K, Huynh W, Horowitz L F, et al. Angew Chem Int Edit, 2016, 55(12):3862
-
[35] Ho C M B, Ng S H, Li K H H, et al. Lab Chip, 2015, 15(18):3627
-
[36] Yazdi A A, Popma A, Wong W, et al. Microfluid Nanofluid, 2016, 20(3):50
-
[37] Zhang L, Wang W, Ju X J, et al. RSC Adv, 2015, 5(8):5638
-
[38] Lupton D. Soc Theory Health, 2015, 13(2):99
-
-

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