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Citation: DAI Jing, GAO Lehong, PENG Fangda, YU Lijia, WANG Chaodong, WANG Yuping, DING Chunguang. Simultaneous determination of twelve antiepileptic drugs in serum by ultra high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2020, 38(8): 900-905. doi: 10.3724/SP.J.1123.2019.12025 shu

Simultaneous determination of twelve antiepileptic drugs in serum by ultra high performance liquid chromatography-tandem mass spectrometry

1.
National Center for Occupational Safety and Health, Beijing 102308, China;
2.
Xuanwu Hospital Capital Medical University, Beijing 100053, China

Corresponding author: DING Chunguang, 13466322180@163.com
Received Date: 18 December 2019

Fund Project: Key Technology Project of State Administration of Work Safety (No. 2017005)

A sensitive, high-throughput method was established for the simultaneous determination of 12 antiepileptics in serum by ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The antiepileptics were gabapentin, lamotrigine, pregabalin, lacosamide, levetiracetam, topiramate, oxcarbazepine, clonazepam, sodium valproate, carbamazepine, phenobarbital and phenytoin sodium. Phenacetin and chlorzoxazone were used as internal standards. The antiepileptics and internal standards were extracted from serum by protein precipitation using acetonitrile as the precipitant. Chromatographic separation was achieved on an ACQUITY UPLC BEH C₁₈ column with a gradient mobile phase comprising 10 mmol/L ammonium formate aqueous solution and methanol (containing 10 mmol/L ammonium formate) at a flow rate of 0.4 mL/min. Detection was performed in multiple reaction monitoring mode with ion mode switching. The results showed good linear trends in their respective concentration ranges and the correlation coefficients (r²) were greater than 0.992. The spiked recoveries of the 12 antiepileptics in serum were 90.80%-114.0% at the three spiked levels. The intra-assay (n=6) and inter-assay (n=3) precisions were less than 13.2% and 14.8%, respectively. The method has high specificity and sensitivity, and it can be used for clinical blood concentration monitoring and pharmacokinetic studies of the 12 antiepileptics.
- ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- blood drug concentration,
- antiepileptics,
- serum
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