Citation: LI Feng, ZHANG Yanmei, KANG Jingwu. Application of capillary electrophoresis in enzyme inhibitors screening from natural products[J]. Chinese Journal of Chromatography, ;2020, 38(5): 502-515. doi: 10.3724/SP.J.1123.2019.12012 shu

Application of capillary electrophoresis in enzyme inhibitors screening from natural products

1.
School of Chemical Engineering, Xi'an University, Xi'an 710065, China;
2.
Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;
3.
State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China

Corresponding author: ZHANG Yanmei, zymei0706@126.com
Received Date: 5 December 2019

Fund Project: Natural Science Foundation of Shaanxi Province (No. 2018JQ2024)

As aberrant enzyme functions are often closely associated with various diseases, enzymes represent one of the most important drug target groups. Historically, natural products have been the most productive sources for drug discovery. However, the complexity of the components leads to a bottleneck in separation and purification, thus restricting activity-based drug screening. Due to the unique advantages such as high separation efficiency and without sample matrix interference, the advantage of capillary electrophoresis (CE) directly applied to extracts without purification for activity-based drug screening is of great attractive. In this review, we have summarized the application of CE for screening enzyme inhibitors in natural products during the last decade. In particular, we discuss important examples for screening inhibitors of transferases (kinases), hydrolases, oxidoreductases, etc. We also present an overview of the technical details concerning the separation modes in CE and enzyme kinetics. Finally, we provide future prospects for the application of CE to the screening of active ingredients in natural products.
- capillary electrophoresis (CE),
- drug screening,
- enzyme inhibitors,
- natural products
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