Citation: FENG Ping, DING Xiaojing, GAO Tie, DU Ruyun, CHEN Hongxu. Determination of A2 β-casein and total β-casein in cow milk and milk powder by capillary zone electrophoresis[J]. Chinese Journal of Chromatography, ;2020, 38(6): 722-729. doi: 10.3724/SP.J.1123.2019.11005 shu

Determination of A2 β-casein and total β-casein in cow milk and milk powder by capillary zone electrophoresis

FENG Ping ^2,, ,
DING Xiaojing ³ ,
GAO Tie ¹ ,
DU Ruyun ⁴ ,
CHEN Hongxu ^1,,

1.
SCIEX China, Beijing 100015, China;
2.
Wyeth Nutrition, Shanghai 200041, China;
3.
Beijing Center for Diseases Prevention and Control, Beijing 100013, China;
4.
Shanghai Institute of Quality Inspection and Technical Research, Shanghai 200233, China

Corresponding author: FENG Ping, CHEN Hongxu,
Received Date: 5 November 2019

β-casein (β-CN) is one of the major casein proteins in cow milk. There are 13 different variants documented for β-CN in cow milk, among which A1 and A2 are the major variants. The separation and quantitation of A2 β-CN are imperative for A2 dairy products. A new capillary zone electrophoresis (CZE) method with UV detection at 214 nm was established for the separation and quantification of the A2 variant and total β-CN content in cow milk and milk powders. The separation of β-CN variants was achieved on bare fused silica capillaries (50 μm×30/40 cm (effective/total length)). The separation buffer was a mixture of 4 mol/L urea, 0.2% (mass fraction) hydroxypropyl methylcellulose, 140 mmol/L citric acid, and 50 mmol/L disodium hydrogen phosphate buffer (pH 2.7). The corrected peak areas and the concentrations of total β-CN and the A2 variant showed good linearity, with correlation coefficients (r²) ranging from 0.9968 to 0.9997. The intra-day precisions for A2 β-CN and total β-CN determination in four samples (two pasteurized milk samples and two milk powder samples) were in the ranges of 2.4%-4.7% and 2.6%-4.8%, respectively. The inter-day precisions for A2 β-CN and total β-CN determination in four samples were in the ranges of 4.0%-6.3% and 3.9%-6.7%, respectively. The recoveries of A2 and total β-CN ranged from 85.5% to 106.4%. With the established CZE method, the A2 β-CN variant and total β-CN protein in liquid and powder bovine milk products could be separated and accurately quantified. By calculating the A2 β-CN content in the total β-CN, the quality of A2 dairy products can be evaluated, and this in turn would aid in the protection of consumer rights.
- capillary zone electrophoresis (CZE),
- A2 β-casein,
- total β-casein,
- liquid milk,
- milk powder
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