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Citation: JIN Yibao, LI Shangfu, WANG Jue, GAO Dan, LIU Hongxia, JIANG Yuyang, YIN Guo, WANG Tiejie. A liquid chromatography-mass spectrometry-based metabolomic study on the changes of intracellular lipid metabolism regulated by Pokemon[J]. Chinese Journal of Chromatography, ;2020, 38(2): 162-168. doi: 10.3724/SP.J.1123.2019.08013 shu

A liquid chromatography-mass spectrometry-based metabolomic study on the changes of intracellular lipid metabolism regulated by Pokemon

JIN Yibao ¹ ,
LI Shangfu ² ,
WANG Jue ¹ ,
GAO Dan ² ,
LIU Hongxia ² ,
JIANG Yuyang ² ,
YIN Guo ¹ ,
WANG Tiejie ^1,,

1.
Shenzhen Institute for Drug Control, Shenzhen Key Laboratory of Drug Quality Standard Research, Shenzhen 518057, China;
2.
Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China

Corresponding author: WANG Tiejie, szyjwtj@163.com
Received Date: 14 August 2019

Fund Project: Chinese Postdoctoral Science Foundation (No. 2015M572370)

Pokemon, a transcriptional repressor, inhibits the transcription of tumor suppressor genes and promotes tumor formation by affecting chromatin recombination or binding directly to the tumor suppressor genes. In this study, the mechanism behind the Pokemon regulation of liver cancer cell metabolism was evaluated using liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. Pokemon-overexpressing HL7702 cells were obtained by lipofection. The cells were collected at different time points after transfection and their intracellular metabolites were analyzed using an LC-MS-based metabolomic method. Based on the results of a multivariate statistical analysis, candidate metabolites with significant differences were selected, and 36 of them were confirmed by database and MS/MS analysis. These metabolites were primarily involved in lipid synthesis by KEGG database searches. Further analysis indicated that both the acetyl-coenzyme carboxylase and the fatty acid synthase were activated in the lipid synthesis pathway. The results show that Pokemon can affect cell metabolism by activating the lipid synthesis pathways in cells.
- liquid chromatography-mass spectrometry (LC-MS),
- Pokemon,
- metabolomics,
- lipid synthesis
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