管内硼亲和固相微萃取-高效液相色谱全自动在线联用检测茶饮料中的顺式二羟基化合物

引用本文: 王欣, 何坚刚, 罗琪, 刘震. 管内硼亲和固相微萃取-高效液相色谱全自动在线联用检测茶饮料中的顺式二羟基化合物[J]. 色谱, 2020, 38(1): 137-142. doi: 10.3724/SP.J.1123.2019.06020 shu

Citation: WANG Xin, HE Jiangang, LUO Qi, LIU Zhen. Automated hyphenation of in-tube boronate affinity solid-phase microextraction with high performance liquid chromatography for the determination of cis-diol compounds in tea beverages[J]. Chinese Journal of Chromatography, 2020, 38(1): 137-142. doi: 10.3724/SP.J.1123.2019.06020 shu

管内硼亲和固相微萃取-高效液相色谱全自动在线联用检测茶饮料中的顺式二羟基化合物

南京大学化学化工学院, 生命分析化学国家重点实验室, 江苏南京 210023

通讯作者: 刘震,E-mail:zhenliu@nju.edu.cn

基金项目:
国家自然科学基金面上项目（21075063）.

摘要: 自动化联用分析技术对于降低人力强度、提高效率和保证数据重现性等具有重要意义。硼亲和固相微萃取（BA-SPME）是近十年出现的用于富集顺式二羟基化合物的独特工具，但BA-SPME与高效液相色谱（HPLC）的自动化在线联用还未见报道。该文报道了一种新颖的管内BA-SPME-HPLC全自动在线联用方法，用于分析茶饮料中的顺式二羟基化合物。该自动化在线联用方法利用自动进样器通过六通阀的切换实现流路连接。制备了管内BA-SPME毛细管，考察了涂层柱的柱容量，并对其形貌进行了表征，考察并优化了影响实际样品分离效果的因素。最后，利用该联用方法对3种不同品牌的茶饮料进行了分析，并对沏茶温度对茶水中顺式二羟基化合物含量的影响进行了评价。

关键词:

English

Automated hyphenation of in-tube boronate affinity solid-phase microextraction with high performance liquid chromatography for the determination of cis-diol compounds in tea beverages

State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China

Corresponding author: LIU Zhen, zhenliu@nju.edu.cn

Received Date: 24 June 2019
Fund Project:
General Grant from the National Natural Science Foundation of China (No. 21075063).

Abstract: Automatic hyphenated analytical techniques hold great promise for reducing labour intensity, enhancing efficiency, and ensuring data reproducibility. In recent decade, Boronate affinity solid phase microextraction (BA-SPME) has emerged as a unique tool for the selective extraction of cis-diol-containing compounds. However, automatic hyphenation of SPME with high performance liquid chromatography (HPLC) has not yet been reported. Herein we report a new automated hyphenation of in-tube BA-SPME with HPLC for the determination of cis-diol compounds in tea beverages. The automated hyphenation was implemented through a flow-switch via the six-way valve of an autosampler. We prepared an in-tube SPME capillary, investigated the loading capacity of the coated column, characterized the morphology of the column, and investigated the factors influencing the separation. Finally, using the automatic hyphenated SPME-HPLC approach, we analyzed the cis-diol-containing compounds in three kinds of commercial tea beverages and investigated the effect of tea-making temperature on the content of the cis-diol-containing compounds in the prepared tea.

Key words:

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管内硼亲和固相微萃取-高效液相色谱全自动在线联用检测茶饮料中的顺式二羟基化合物

通讯作者: 刘震,E-mail:zhenliu@nju.edu.cn

English

Automated hyphenation of in-tube boronate affinity solid-phase microextraction with high performance liquid chromatography for the determination of cis-diol compounds in tea beverages

Corresponding author: LIU Zhen, zhenliu@nju.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

管内硼亲和固相微萃取-高效液相色谱全自动在线联用检测茶饮料中的顺式二羟基化合物

通讯作者: 刘震,E-mail:zhenliu@nju.edu.cn

English

Automated hyphenation of in-tube boronate affinity solid-phase microextraction with high performance liquid chromatography for the determination of cis-diol compounds in tea beverages

Corresponding author: LIU Zhen, zhenliu@nju.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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