功能化磁性纳米材料在磷酸化肽富集中的应用

引用本文: 熊芳芳, 江丹丹, 贾琼. 功能化磁性纳米材料在磷酸化肽富集中的应用[J]. 色谱, 2020, 38(1): 60-65. doi: 10.3724/SP.J.1123.2019.06019 shu

Citation: XIONG Fangfang, JIANG Dandan, JIA Qiong. Application of functionalized magnetic nanomaterials in phosphopeptide enrichment[J]. Chinese Journal of Chromatography, 2020, 38(1): 60-65. doi: 10.3724/SP.J.1123.2019.06019 shu

功能化磁性纳米材料在磷酸化肽富集中的应用

吉林大学化学学院, 吉林长春 130012
基金项目:
吉林大学超分子结构与材料国家重点实验室开放课题（sklssm2019020）.

摘要: 蛋白质磷酸化是最重要和最普遍的翻译后修饰之一。基于质谱的技术已成为分析蛋白质磷酸化的重要手段。然而，磷酸化肽固有的低丰度和电离效率以及由非磷酸化肽共存引起的严重抑制使得直接质谱分析仍然是一个挑战。为解决此问题，需在质谱分析前对磷酸化蛋白质进行选择性富集。磁性纳米材料具有良好的磁响应性，可以在外界磁铁的帮助下实现与溶液的迅速分离。功能化磁性纳米材料作为一种新型的分析技术已在蛋白质组学研究中得到广泛的应用。该文就近年来对磁性纳米粒子进行各种功能化修饰以提高其特异性吸附能力的吸附材料在磷酸化肽的富集方面的应用予以综述，并展望了功能化磁性纳米材料在磷酸化肽富集领域的应用前景。

关键词:

功能化
/ 磁性
/ 磷酸化肽
/ 综述

English

Application of functionalized magnetic nanomaterials in phosphopeptide enrichment

College of Chemistry, Jilin University, Changchun 130012, China
Received Date: 24 June 2019
Fund Project:
Open Project of State Key Laboratory of Supramolecular Structure and Materials, Jilin University (No.sklssm201815).

Abstract: Protein phosphorylation is one of the most important and prevalent post-translational modifications. Mass spectrometry (MS) has become a powerful tool for the analysis of phosphopeptides. However, the low dynamic stoichiometry, poor ionization efficiency, and signal suppression by high-abundance non-phosphorylated peptides have hindered the direct applications in the analysis of phosphopeptides. Therefore, the development of highly selective and sensitive enrichment strategies is necessary for the identification of phosphopeptides prior to MS analysis. Magnetic nanomaterials have good magnetic responsiveness and can be separated rapidly from the solution with the help of external magnets. Functionalized magnetic nanomaterials have been widely used as a new analytical tool in proteomics research. This review surveys recently reported functionalized magnetic nanoparticles for the enrichment of phosphopeptides reported in recent years. Further trends in the enrichment of phosphopeptides with functionalized magnetic materials are also prospected.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

功能化磁性纳米材料在磷酸化肽富集中的应用

English

Application of functionalized magnetic nanomaterials in phosphopeptide enrichment

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通讯作者: 陈斌, bchen63@163.com

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