分子印迹技术在蛋白质分离分析中的研究进展

孙晓宇 马润恬 师彦平

引用本文: 孙晓宇,  马润恬,  师彦平. 分子印迹技术在蛋白质分离分析中的研究进展[J]. 色谱, 2020, 38(1): 50-59. doi: 10.3724/SP.J.1123.2019.06017 shu
Citation:  SUN Xiaoyu,  MA Runtian,  SHI Yanping. Recent advances in molecular imprinting technology for the separation and analysis of proteins[J]. Chinese Journal of Chromatography, 2020, 38(1): 50-59. doi: 10.3724/SP.J.1123.2019.06017 shu

分子印迹技术在蛋白质分离分析中的研究进展

  • 基金项目:

    国家自然科学基金项目(21575150,21775153,21804135);中国科学院"西部之光"人才培养引进计划-西部青-学者项目(B类).

摘要: 蛋白质结构复杂,种类多样,与各种生命活动密切相关。大部分蛋白质在生物体中含量极低,对其分析检测带来极大困难。因此实现复杂生物样品中蛋白质的选择性识别与分离,对实现蛋白质的分离分析意义重大。通过分子印迹技术制备的分子印迹聚合物含有与模板分子大小、形状一致,官能团相互匹配的三维印迹空穴,在蛋白质的选择性识别与分离领域显示出了巨大的发展潜力。但是,由于蛋白质具有尺寸较大、构型易变、结构复杂等特点,分子印迹技术在蛋白质印迹中面临着巨大挑战。该文在介绍几种新型分子印迹技术包括表面印迹、抗原决定基印迹和金属螯合物印迹的基础上,综述了近3年分子印迹技术在蛋白质分离分析方面的应用,并对其发展进行了总结与展望。

English

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  • 收稿日期:  2019-06-19
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