分子印迹技术在蛋白质分离分析中的研究进展

引用本文: 孙晓宇, 马润恬, 师彦平. 分子印迹技术在蛋白质分离分析中的研究进展[J]. 色谱, 2020, 38(1): 50-59. doi: 10.3724/SP.J.1123.2019.06017 shu

Citation: SUN Xiaoyu, MA Runtian, SHI Yanping. Recent advances in molecular imprinting technology for the separation and analysis of proteins[J]. Chinese Journal of Chromatography, 2020, 38(1): 50-59. doi: 10.3724/SP.J.1123.2019.06017 shu

分子印迹技术在蛋白质分离分析中的研究进展

1.
中国科学院兰州化学物理研究所, 中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室, 甘肃兰州 730000;
2.
中国科学院大学, 北京 100049

通讯作者: 师彦平,E-mail:shiyp@licp.cas.cn

基金项目:
国家自然科学基金项目（21575150，21775153，21804135）；中国科学院"西部之光"人才培养引进计划-西部青-学者项目（B类）.

摘要: 蛋白质结构复杂，种类多样，与各种生命活动密切相关。大部分蛋白质在生物体中含量极低，对其分析检测带来极大困难。因此实现复杂生物样品中蛋白质的选择性识别与分离，对实现蛋白质的分离分析意义重大。通过分子印迹技术制备的分子印迹聚合物含有与模板分子大小、形状一致，官能团相互匹配的三维印迹空穴，在蛋白质的选择性识别与分离领域显示出了巨大的发展潜力。但是，由于蛋白质具有尺寸较大、构型易变、结构复杂等特点，分子印迹技术在蛋白质印迹中面临着巨大挑战。该文在介绍几种新型分子印迹技术包括表面印迹、抗原决定基印迹和金属螯合物印迹的基础上，综述了近3年分子印迹技术在蛋白质分离分析方面的应用，并对其发展进行了总结与展望。

关键词:

分子印迹
/ 蛋白质
/ 分离分析
/ 综述

English

Recent advances in molecular imprinting technology for the separation and analysis of proteins

1.
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
2.
University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: SHI Yanping, shiyp@licp.cas.cn

Received Date: 19 June 2019
Fund Project:
National Natural Science Foundation of China (Nos. 21575150, 21775153, 21804135)

Abstract: Proteins, which have complex structures and fall under diverse categories, are closely associated with different kinds of life activities. Majority of the proteins exist in low concentrations in complex biological samples, because of which their direct analysis without sample pretreatment is difficult. Thus, selective separation of the target proteins from complex biological samples is essential for efficient analysis. Molecularly imprinted polymers, containing numerous imprinted cavities that are complementary with the templates in terms of shape, size, and functional groups, have great potential for use in the selective recognition and separation of the target proteins from complex biological samples. However, the large size, structural flexibility, and complex structures of proteins hinder their efficient separation and analysis by conventional molecular imprinting technology. In this review, several novel molecular imprinting techniques, including surface imprinting, epitope imprinting, and metal chelate imprinting, are introduced. The emergence of these novel technologies has contributed to advances in protein analysis. The applications of these molecular imprinting techniques to the separation and analysis of proteins in the last three years are summarized herein. Finally, the promising future of molecular imprinting techniques in the area of proteins is prospected.

Key words:

1. [1] Dinc M, Esen C, Mizaikoff B. TrAC-Trends Anal Chem, 2019, 114:202
2. [2] Huang S, Xu J, Zheng J, et al. Anal Bioanal Chem, 2018, 410(17):3991
3. [3] Ben Aissa A, Herrera-Chacon A, Pupin R R, et al. Biosens Bioelectron, 2017, 88:101
4. [4] Shi W, Zhang S-Q, Li K-B, et al. Sep Purif Technol, 2018, 202:165
5. [5] Pan J, Chen W, Ma Y, et al. Chem Soc Rev, 2018, 47(15):5574
6. [6] Xu J, Prost E, Haupt K, et al. Sensor Actuat B-Chem, 2018, 258:10
7. [7] Wang X, Yu J, Li J, et al. Sensor Actuat B-Chem, 2018, 255:268
8. [8] Zhang X, Yang S, Jiang R, et al. Sensor Actuat B-Chem, 2018, 254:1078
9. [9] Zhang Z, Li Y, Zhang X, et al. Nanoscale, 2019, 11(11):4854
10. [10] Ma R T, Shi Y P. Chinese Journal of Chromatography, 2016, 34(3):223 马润恬, 师彦平. 色谱, 2016, 34(3):223
11. [11] Peng S, Li H, Shi S Y. Chinese Journal of Chromatography, 2019, 37(3):293 彭胜, 李奂, 施树云. 色谱, 2019, 37(3):293
12. [12] Ansari S, Masoum S. TrAC-Trends Anal Chem, 2019, 114:29
13. [13] Wang X, Yu S, Liu W, et al. ACS Sensors, 2018, 3(2):378
14. [14] Hou H Q, Su L M, Huang Y Y, et al. Chinese Journal of Chromatography, 2016, 34(12):1206 侯会卿, 苏黎明, 黄嫣嫣, 等. 色谱, 2016, 34(12):1206
15. [15] Chrzanowska A M, Poliwoda A, Wieczorek P P. J Chromatogr A, 2015, 1392:1
16. [16] Peng M, Xiang H, Hu X, et al. J Chromatogr A, 2016, 1474:8
17. [17] Sun Z A, Qi Y X, Wang X, et al. Chinese Journal of Chromatography, 2018, 36(8):176 孙治安, 祁玉霞, 王霞, 等. 色谱, 2018, 36(8):176
18. [18] Zhang W, Han Y, Chen X, et al. Food Chem, 2017, 232:145
19. [19] Sonawane S L, Asha S K. J Polym Sci Pol Chem, 2017, 55(9):1558
20. [20] Wang H, Liu Y, Yao S, et al. Food Chem, 2018, 240:1262
21. [21] Ning F, Qiu T, Wang Q, et al. Food Chem, 2017, 221:1797
22. [22] Qiu H T, Zheng N N, Fang Q H, et al. Chinese Journal of Chromatography, 2019 37(7):692 邱昊田, 郑宁宁, 方权辉, 等. 色谱, 2019 37(7):692
23. [23] Wang S, Ye J, Bie Z, et al. Chem Sci, 2014, 5(3):1135
24. [24] Fan H, Wang J, Meng Q, et al. Food Chem, 2019, 281:1
25. [25] Hua S, Zhao L, Cao L, et al. Chem Eng J, 2018, 345:414
26. [26] Huang W, Hou X, Tong Y, et al. RSC Adv, 2019, 9(10):5394
27. [27] Bie Z, Chen Y, Ye J, et al. Angew Chem Int Edit, 2015, 54(35):10211
28. [28] Zhao X-L, Li D-Y, He X-W, et al. J Mater Chem B, 2014, 2(43):7575
29. [29] Li W, Sun Y, Yang C, et al. ACS Appl Mater Inter, 2015, 7(49):27188
30. [30] Demirci B, Bereli N, Asliyuce S, et al. J Sep Sci, 2017, 40(7):1610
31. [31] Xu W, Wang Y, Wei X, et al. Anal Chim Acta, 2019, 1048:1
32. [32] Zhang Z, Zhang X, Niu D, et al. J Mate Chem B, 2017, 5(22):4214
33. [33] Zhou J, Wang Y, Ma Y, et al. Appl Surf Sci, 2019, 486:265
34. [34] Fan J-P, Yu J-X, Yang X-M, et al. Chem Eng J, 2018, 337:722
35. [35] Qin Y P, Wang H Y, He X W, et al. Talanta, 2018, 185:620
36. [36] Bakhshpour M, Tamahkar E, Andac M, et al. Colloid Surface B, 2017, 158:453
37. [37] Dolak I, Canpolat G, Ersöz A, et al. Sep Sci Technol, 2019:1
38. [38] Li Y Y, Zhang X, Chen W J, et al. Chinese Journal of Chromatography, 2019, 37(4):392 李园园, 张鑫, 陈炜杰, 等. 色谱, 2019, 37(4):392
39. [39] Lan F, Ma S, Ma J, et al. Mat Sci Eng C Mater, 2017, 70:1076
40. [40] Zhang Z, Wang H, Wang H, et al. Analyst, 2018, 143(23):5849
41. [41] Xu K, Wang Y, Wei X, et al. Microchim Acta, 2018, 185(2):146
42. [42] Xu X, Guo P, Luo Z, et al. RSC Adv, 2017, 7(30):18765
43. [43] Xie X, Hu Q, Ke R, et al. Chem Eng J, 2019, 371:130
44. [44] Zhang Z, Li L. J Sep Sci, 2018, 41(11):2479
45. [45] Wang Y, Yang C, Sun Y, et al. J Sep Sci, 2018, 41(3):765
46. [46] Su Y, Qiu B, Chang C, et al. RSC Adv, 2018, 8(11):6192
47. [47] Li Y, Chen Y, Huang L, et al. Analyst, 2017, 142(2):302
48. [48] Wang H, Ying X, Liu J, et al. J Polym Res, 2017, 24(6):93
49. [49] Boitard C, Rollet A L, Menager C, et al. Chem Commun, 2017, 53(63):8846
50. [50] Shoghi E, Mirahmadi-Zare S Z, Ghasemi R, et al. Microchim Acta, 2018, 185(4):241
51. [51] Qian L, Sun J, Hou C, et al. Talanta, 2017, 168:174
52. [52] Xu X, Liu R, Guo P, et al. Food Chem, 2018, 256:91
53. [53] Li Z, Guan P, Hu X, et al. Polymers, 2018, 10(3):298
54. [54] Zhang X, Zhang N, Du C, et al. Chem Eng J, 2017, 317:988
55. [55] Bagán H, Zhou T, Eriksson N L, et al. RSC Adv, 2017, 7(66):41705
56. [56] Çimen D, Bereli N, Andaç M, et al. Sep Sci Plus, 2018, 1(6):454
57. [57] Gong X, Tang B, Liu J J, et al. Biosens Bioelectron, 2017, 87:858
58. [58] Yang F, Deng D, Dong X, et al. J Chromatogr A, 2017, 1494:18
59. [59] Qin Y P, Jia C, He X W, et al. ACS Appl Mater Inter, 2018, 10(10):9060
60. [60] Luo J, Huang J, Cong J, et al. ACS Appl Mater Inter, 2017, 9(8):7735
61. [61] Bie Z, Xing R, He X, et al. Anal Chem, 2018, 90(16):9845
62. [62] Xie J, Zhong G, Cai C, et al. Talanta, 2017, 169:98
63. [63] Zhu H, Yao H, Xia K, et al. Chem Eng J, 2018, 346:317
64. [64] Ma R-T, Ha W, Chen J, et al. J Mater Chem B, 2016, 4(15):2620
65. [65] Sun X Y, Ma R T, Chen J, et al. Microchim Acta, 2018, 185(12):565
66. [66] Li D, Tu T, Yang M, et al. Talanta, 2018, 184:316
67. [67] Wang P, Zhu H, Liu J, et al. Chem Eng J, 2019, 358:143
68. [68] Sun X-Y, Ma R-T, Chen J, et al. J Mater Chem B, 2018, 6(4):688
69. [69] He P, Zhu H, Ma Y, et al. Chem Eng J, 2019, 367:55
70. [70] Sun X-Y, Ma R-T, Chen J, et al. Microchim Acta, 2017, 184(10):3729
71. [71] Ma R-T, Sun X-Y, Ha W, et al. J Mater Chem B, 2017, 5(36):7512

扫一扫看文章

计量

PDF下载量: 12
文章访问数: 473
HTML全文浏览量: 76

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

分子印迹技术在蛋白质分离分析中的研究进展

通讯作者: 师彦平,E-mail:shiyp@licp.cas.cn

English

Recent advances in molecular imprinting technology for the separation and analysis of proteins

Corresponding author: SHI Yanping, shiyp@licp.cas.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

分子印迹技术在蛋白质分离分析中的研究进展

通讯作者: 师彦平,E-mail:shiyp@licp.cas.cn

English

Recent advances in molecular imprinting technology for the separation and analysis of proteins

Corresponding author: SHI Yanping, shiyp@licp.cas.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs