分子印迹技术在蛋白质分离分析中的研究进展

引用本文: 孙晓宇, 马润恬, 师彦平. 分子印迹技术在蛋白质分离分析中的研究进展[J]. 色谱, 2020, 38(1): 50-59. doi: 10.3724/SP.J.1123.2019.06017 shu

Citation: SUN Xiaoyu, MA Runtian, SHI Yanping. Recent advances in molecular imprinting technology for the separation and analysis of proteins[J]. Chinese Journal of Chromatography, 2020, 38(1): 50-59. doi: 10.3724/SP.J.1123.2019.06017 shu

分子印迹技术在蛋白质分离分析中的研究进展

1.
中国科学院兰州化学物理研究所, 中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室, 甘肃兰州 730000;
2.
中国科学院大学, 北京 100049
基金项目:
国家自然科学基金项目（21575150，21775153，21804135）；中国科学院"西部之光"人才培养引进计划-西部青-学者项目（B类）.

摘要: 蛋白质结构复杂，种类多样，与各种生命活动密切相关。大部分蛋白质在生物体中含量极低，对其分析检测带来极大困难。因此实现复杂生物样品中蛋白质的选择性识别与分离，对实现蛋白质的分离分析意义重大。通过分子印迹技术制备的分子印迹聚合物含有与模板分子大小、形状一致，官能团相互匹配的三维印迹空穴，在蛋白质的选择性识别与分离领域显示出了巨大的发展潜力。但是，由于蛋白质具有尺寸较大、构型易变、结构复杂等特点，分子印迹技术在蛋白质印迹中面临着巨大挑战。该文在介绍几种新型分子印迹技术包括表面印迹、抗原决定基印迹和金属螯合物印迹的基础上，综述了近3年分子印迹技术在蛋白质分离分析方面的应用，并对其发展进行了总结与展望。

关键词:

分子印迹
/ 蛋白质
/ 分离分析
/ 综述

English

Recent advances in molecular imprinting technology for the separation and analysis of proteins

1.
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
2.
University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China
Received Date: 19 June 2019
Fund Project:
National Natural Science Foundation of China (Nos. 21575150, 21775153, 21804135)

Abstract: Proteins, which have complex structures and fall under diverse categories, are closely associated with different kinds of life activities. Majority of the proteins exist in low concentrations in complex biological samples, because of which their direct analysis without sample pretreatment is difficult. Thus, selective separation of the target proteins from complex biological samples is essential for efficient analysis. Molecularly imprinted polymers, containing numerous imprinted cavities that are complementary with the templates in terms of shape, size, and functional groups, have great potential for use in the selective recognition and separation of the target proteins from complex biological samples. However, the large size, structural flexibility, and complex structures of proteins hinder their efficient separation and analysis by conventional molecular imprinting technology. In this review, several novel molecular imprinting techniques, including surface imprinting, epitope imprinting, and metal chelate imprinting, are introduced. The emergence of these novel technologies has contributed to advances in protein analysis. The applications of these molecular imprinting techniques to the separation and analysis of proteins in the last three years are summarized herein. Finally, the promising future of molecular imprinting techniques in the area of proteins is prospected.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

分子印迹技术在蛋白质分离分析中的研究进展

English

Recent advances in molecular imprinting technology for the separation and analysis of proteins

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