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Citation: HAN Runchuan, BAN Yanna, ZHEN Qianna, ZHOU Ying, ZHANG Xiaoqing, DING Min. Determination of indoles in plasma of polycystic ovarian syndrome patients by high performance liquid chromatography-fluorescence detection[J]. Chinese Journal of Chromatography, ;2019, 37(12): 1337-1342. doi: 10.3724/SP.J.1123.2019.05039 shu

Determination of indoles in plasma of polycystic ovarian syndrome patients by high performance liquid chromatography-fluorescence detection

1.
Key Laboratory of Clinical Laboratory Dignostics(Ministry of Education of China), School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China;
2.
Reproductive Health and Infertility Department the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;
3.
Endocrinology Department, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

Received Date: 23 May 2019

Fund Project: Youth Project of Science and Technology Research Program of Chongqing Education Commission of China (No. 2018-9-76)

A new method was established for the determination of indoles (indole (IND), 3-methylindole (3-MI), indolyl-3-acetic acid (IAA) and indolyl-3-propionic acid (IPA)) in plasma by high performance liquid chromatography-fluorescence detection (HPLC-FLD). The analytes were separated simultaneously on a Shim-pack VP-ODS column (150 mm×4.6 mm, 4.6 μm) using 15 mmol/L sodium dihydrogen phosphate solution and methanol (48:52, v/v) as the mobile phases. The column temperature was 30 ℃, and the flow rate was 0.8 mL/min. The calibration curves of IND, 3-MI, IPA and IAA showed good linear relationships. The intra-day and inter-day relative standard deviations (RSDs) for the analytes were both less than 6.31%. The average recoveries of the analytes in plasma were 97.5%-107.0%. The method was successfully applied to the analysis of indoles in the plasma of healthy women of reproductive age (n=25) as controls and patients with polycystic ovarian syndrome (n=61). The results showed that the concentrations of indoles in the plasma were significantly different between the two groups, and IND was found to be a risk factor and a potential diagnostic biomarker for polycystic ovarian syndrome (PCOS). The method is simple, sensitive and suitable for use in clinical testing and laboratory research.
- high performance liquid chromatography-fluorescence detection (HPLC-FLD),
- polycystic ovarian syndrome (PCOS),
- indoles,
- plasma
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