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Citation: WANG Wei, HE Tianyu, LAN Tao, XI Xingjun, ZHAO Xinying. Isolation and preparation of polyphenols from grape seed by high-speed counter-current chromatography combined with preparative high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2019, 37(11): 1193-1199. doi: 10.3724/SP.J.1123.2019.05024 shu

Isolation and preparation of polyphenols from grape seed by high-speed counter-current chromatography combined with preparative high performance liquid chromatography

WANG Wei ^1, ,
HE Tianyu ¹ ,
LAN Tao ² ,
XI Xingjun ² ,
ZHAO Xinying ¹

1.
Beijing Center for Physical and Chemical Analysis, Beijing Key Laboratory of Organic Materials Testing Technology & Quality Evaluation, Beijing 100094, China;
2.
China National Institute of Standardization, Beijing 100191, China

Received Date: 16 May 2019

Fund Project: National Key Research and Development Program of China (No. 2017YFF0207800).

Eight polyphenols were separated from grape seed by high-speed counter-current chromatography (HSCCC). The separation was performed using a two-phase solvent system composed of 1-butanol-ethyl-water (1:14:15, v/v/v) and n-hexane-ethyl-water (1:10:10, v/v/v). The upper and lower phases were used as the stationary and mobile phases, respectively. A flow rate of 2.0 mL/min was employed for the separation. The apparatus was rotated at 900 r/min, and the detection wavelength was set at 280 nm. Under the selected conditions, procyanidins B₁, procyanidins B₂, gallic acid, epicatechin gallate, and catechin were obtained from the grape seed extracts with a purity of 98.5%, 97.2%, 98.3%, 98.9%, and 96.7%, respectively. Epicatechin, epicatechin gallate, and gallocatechin gallate were obtained by preparative high performance liquid chromatography with the purity of 99.2%, 99.3%, and 99.2%, respectively. The proposed method is simple and shows high separation efficiency, and will be of great importance for the comprehensive utilization of grape seed.
- high-speed counter-current chromatography (HSCCC),
- polyphenol,
- procyanidins,
- catechin,
- grape seed
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