气相色谱正构烷烃和直链脂肪酸甲酯两种线性保留指数之间的转换方法

引用本文: 李勇, 逄涛, 师君丽, 邓小鹏, 孔光辉, 卢秀萍. 气相色谱正构烷烃和直链脂肪酸甲酯两种线性保留指数之间的转换方法[J]. 色谱, 2020, 38(2): 244-249. doi: 10.3724/SP.J.1123.2019.05018 shu

Citation: LI Yong, PANG Tao, SHI Junli, DENG Xiaopeng, KONG Guanghui, LU Xiuping. Conversion method for linear retention indices in gas chromatography acquired using n-alkanes and n-fatty acid methyl esters[J]. Chinese Journal of Chromatography, 2020, 38(2): 244-249. doi: 10.3724/SP.J.1123.2019.05018 shu

气相色谱正构烷烃和直链脂肪酸甲酯两种线性保留指数之间的转换方法

云南省烟草农业科学研究院, 云南玉溪 653100

通讯作者: 逄涛, E-mail:baggiopt@126.com

基金项目:
中国烟草总公司云南省公司项目（2018530000241007，2018530000241027）.

摘要: 保留指数是气相色谱中用于化合物结构鉴定的重要工具。本研究对基于正构烷烃和直链脂肪酸甲酯的线性保留指数进行线性拟合，发现在所设定的4种不同的升温条件下两种保留指数之间呈现较为固定的线性关系：在弱极性柱（固定液为5%苯基和95%的甲基聚硅氧烷）和极性柱（固定液为聚乙二醇）条件下，正构烷烃保留指数（y）与脂肪酸甲酯保留指数（x）之间的关系分别为y=1.0051x+318.51（r²=1）和y=1.0362x+562.519（r²=1）。采用文献保留指数对所建立的线性关系进行验证，发现通过公式换算得到的保留指数均在其对应文献值的均值加减方差范围内，说明换算公式真实有效。两种保留指数之间关系的建立对于化合物结构鉴定时扩大保留指数搜索范围、减少保留指数测定实验具有重要意义。

关键词:

English

Conversion method for linear retention indices in gas chromatography acquired using n-alkanes and n-fatty acid methyl esters

Yunnan Academy of Tobacco Agricultural Sciences, Yuxi 653100, China

Corresponding author: PANG Tao, baggiopt@126.com

Received Date: 14 May 2019
Fund Project:
China National Tobacco Corporation Yunnan Branch Supported Projects (Nos. 2018530000241007, 2018530000241027).

Abstract: Retention index (RI) is a powerful tool for gas chromatography-based structure elucidation of various compounds. In this study, linear RIs based on n-alkanes and n-fatty acid methyl esters in different temperature programs were fitted. Nearly identical first-order linearities were acquired with different temperature programs. The linear equations were y=1.0051x+318.51 (r²=1) and y=1.0362x+562.519 (r²=1) for polar (packed with polyethylene glycol) and semi-standard non-polar (packed with 5% diphenyl/95% dimethylpolysiloxane) columns, respectively. The variables x and y in the equations stand for RIs based on n-fatty acid methyl esters and n-alkanes, respectively. The established linearity was then verified using the reference RIs collected in the NIST mass spectral library, Golm metabolite library, and Fiehnmetabolite library. The RI values converted using both the acquired equations finely matched those in the references. The established relationship between the two kinds of RIs can be used to expand the RI references and decrease the number of RI acquisition experiments.

Key words:

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气相色谱正构烷烃和直链脂肪酸甲酯两种线性保留指数之间的转换方法

通讯作者: 逄涛, E-mail:baggiopt@126.com

English

Conversion method for linear retention indices in gas chromatography acquired using n-alkanes and n-fatty acid methyl esters

Corresponding author: PANG Tao, baggiopt@126.com

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

气相色谱正构烷烃和直链脂肪酸甲酯两种线性保留指数之间的转换方法

通讯作者: 逄涛, E-mail:baggiopt@126.com

English

Conversion method for linear retention indices in gas chromatography acquired using n-alkanes and n-fatty acid methyl esters

Corresponding author: PANG Tao, baggiopt@126.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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