Citation: ZHANG Wenhuan, LIU Pingxiang, QIU Jing, JIA Qi, QIAN Yongzhong. Rapid simultaneous determination of 10 nutrients in ginger by ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2019, 37(10): 1105-1111. doi: 10.3724/SP.J.1123.2019.03024 shu

Rapid simultaneous determination of 10 nutrients in ginger by ultra-high performance liquid chromatography-tandem mass spectrometry

Institute of Quality Standard and Testing Technology for Agro-Products of Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, China

Received Date: 20 March 2019

Fund Project: National Specialty Vegetable Industry Technology System Post Expert Task (No. CARS-24-E-04).

An analytical method by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed for the simultaneous determination of the two types of nutrients, viz. gingerol-related compounds and curcuminoids in ginger, including 6-gingerol, 8-gingerol, 10-gingerol, 6-shogaol, 8-shogaol, 10-shogaol, tetrahydrocurcumin, curcumin, demethoxycurcumin and bisdemethoxycurcumin. The 10 target compounds were separated on a ZORBAX RRHD Eclipse Plus C18 column (100 mm×2.1 mm, 1.8 μm) using gradient elution with 0.1% (v/v) formic acid aqueous solution and methanol containing 0.1% (v/v) formic acid as the mobile phases. Qualitative confirmation and quantitative analysis of the target compounds were performed using an electrospray ionization (ESI) source in the positive ion and multiple reaction monitoring (MRM) modes. The linear correlation coefficients (r) of the 10 nutrients were ≥ 0.9995. The limits of quantification were 0.10-7.71 μg/L. The average spiked recoveries of the samples at the three levels were 82.8%-115.3%, the relative standard deviations (RSDs) were 0.58%-11.49%. The results showed that all the 10 nutrients in ginger were detected, and the content of 6-gingerol was the highest at 373.35-702.48 mg/kg. This method is convenient, rapid, accurate and reliable, and is suitable for the analysis of gingerol-related and curcuminoids in ginger. It provides a technical means for the quality identification and control of ginger.
- ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS),
- gingerol,
- shogaol,
- curcumin,
- nutrients,
- ginger
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