顶空气相色谱法快速测定污水中氨氮含量

引用本文: 彭容, 刘浩, 柴欣生, 蒋然. 顶空气相色谱法快速测定污水中氨氮含量[J]. 色谱, 2019, 37(10): 1124-1128. doi: 10.3724/SP.J.1123.2019.03001 shu

Citation: PENG Rong, LIU Hao, CHAI Xinsheng, JIANG Ran. Rapid determination of ammonia nitrogen in sewage by headspace gas chromatography[J]. Chinese Journal of Chromatography, 2019, 37(10): 1124-1128. doi: 10.3724/SP.J.1123.2019.03001 shu

顶空气相色谱法快速测定污水中氨氮含量

彭容 ^1, ,
刘浩 ^1, ,
柴欣生 ^1, ,
蒋然 ^2,

1.
华南理工大学制浆造纸工程国家重点实验室, 广东广州 510641;
2.
珠江水利委员会珠江水利科学研究所, 广东广州 510611
基金项目:
广州市科技计划产学研协同创新重大专项（201704020038）；广西创新驱动发展专项（AA18118013-9）.

摘要: 研究了一种基于化学反应型顶空气相色谱的快速检测污水氨氮含量新方法。首先在顶空瓶中用过量的甲醛与NH₄⁺反应产生等物质的量的强酸，再用NaHCO₃与新生成的酸反应产生CO₂，最后经过气相色谱定量CO₂，间接得出样品中氨氮的含量。结果表明，氨氮含量与气相色谱信号值之间存在良好的线性相关性，相关系数为0.999，定量限为0.786 mg/L（以N的质量浓度计），相对标准偏差小于2%，加标回收率在95%~105%之间。与国标纳氏试剂法相比，该方法测定结果的相对偏差在±5%以内。该方法检测效率高，适用于快速批量检测污水中氨氮的含量。

关键词:

English

Rapid determination of ammonia nitrogen in sewage by headspace gas chromatography

1.
State Key Lab of Pulp & Paper Engineering, South China University of Technology, Guangzhou 510641, China;
2.
Pearl River Water Resources Institute, Pear River Water Resources Commission, Guangzhou 510611, China
Received Date: 01 March 2019
Fund Project:
Guangzhou Science and Technology Plan, a Major Project of Industry-University-Research Collaborative Innovation (No. 201704020038)

Abstract: This paper reports a novel method for the rapid determination of ammonia nitrogen in sewage by headspace gas chromatography (HS-GC) through a chemical reaction. During the experiment, formaldehyde, initially in excess, was reacted with NH₄⁺ in a headspace bottle to produce a strong acid with equal molarity. Thereafter, NaHCO₃ was reacted with the newly generated acid to produce CO₂. In the final step, the released CO₂ was determined using a gas chromatograph equipped with a thermal conductivity detector. Good linear correlation between the ammonia nitrogen content and the gas chromatographic signals was achieved. The observed correlation coefficient was 0.999, and the limit of quantification (LOQ) was 0.786 mg/L (measured based on the mass concentration of N). The relative standard deviation of reproducibility was less than 2%, and the standard recovery was between 95% and 105%. Compared with Nassar's reagent method, the relative deviation of this method is within ±5%. This method has high detection efficiency and is suitable for the rapid batch detection of ammonia nitrogen in sewage.

Key words:

1. [1] Zhou L, Boyd C E. Aquaculture, 2015, 437:263
2. [2] Kong J. China Resources Comprehensive Utilization, 2018, 36(4):83 孔杰. 中国资源综合利用, 2018, 36(4):83
3. [3] Yang Y Z, Wang T, Ma W P. Shanxi Architecture, 2010(20):356 杨玉珍, 王婷, 马文鹏. 山西建筑, 2010(20):356
4. [4] Liu W, Wang Z, Zhang Q, et al. Biogeochemistry, 2015, 123(3):379
5. [5] Marzadri A, Dee M M, Tonina D, et al. Proc Natl Acad Sci U S A, 2017, 114(17):4330
6. [6] Yu L Y, Zhao H H, Zhang X S. West Leather, 2010, 32(5):27 俞凌云, 赵欢欢, 张新申. 西部皮革, 2010, 32(5):27
7. [7] Huang Y, Zhang S B. Sichuan Environment, 2016, 35(3):23 黄亿, 张绍斌. 四川环境, 2016, 35(3):23
8. [8] Feng Y P, Qiu H N, Sun Z J. Environmental Science & Technology, 2016(S2):348 封跃鹏, 邱赫男, 孙自杰. 环境科学与技术, 2016(S2):348
9. [9] Huang T Q, Liu Y R. Environmental Monitoring in China, 2003(4):43 黄图强, 刘艳蓉. 中国环境监测, 2003(4):43
10. [10] Haken J K. J Chromatogr A, 1984, 301:309
11. [11] Wang X Y, Guo X Q, Wang Y F, et al. Journal of Capital Normal University (Natural Science Edition), 2006, 27(1):48 王欣月, 郭雪清, 王英锋, 等. 首都师范大学学报(自然科学版), 2006, 27(1):48
12. [12] Chai X S, Fu S Y, Mo S H, et al. Progress in Chemistry, 2008(5):762 柴欣生, 付时雨, 莫淑欢, 等. 化学进展, 2008(5):762
13. [13] Chai X S, Luo Q, Zhu J Y. J Chromatogr A, 2001, 909(2):249
14. [14] Yuan Y, Guo Y F, Wu W, et al. Industrial Water Treatment, 2014, 34(10):73 袁砚, 郭永福, 吴伟, 等. 工业水处理, 2014, 34(10):73
15. [15] Liu Y, Li Y, Chen P H, et al. University Chemistry, 2015, 30(1):45 刘越, 李琰, 陈珮豪, 等. 大学化学, 2015, 30(1):45
16. [16] Ministry of Environmental Protection. No. 77 Ministry of Environmental Protection of the People's Republic of China. (2009-12-31)[2010-04-01]. http://kjs.mee.gov.cn/hjbhbz/bzwb/jcffbz/201001/W020180319535685015821.pdf 环境保护部. 中华人民共和国环境保护部公号第77号. (2009-12-31)[2010-04-01]. http://kjs.mee.gov.cn/hjbhbz/bzwb/jcffbz/201001/W020180319535685015821.pdf

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顶空气相色谱法快速测定污水中氨氮含量

English

Rapid determination of ammonia nitrogen in sewage by headspace gas chromatography

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顶空气相色谱法快速测定污水中氨氮含量

English

Rapid determination of ammonia nitrogen in sewage by headspace gas chromatography

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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