微热助吹扫捕集-超高压液相色谱-串联质谱法快速测定蔬菜中12种半挥发性农药残留

引用本文: 陈正毅, 李运达, 张卓旻, 李攻科. 微热助吹扫捕集-超高压液相色谱-串联质谱法快速测定蔬菜中12种半挥发性农药残留[J]. 色谱, 2020, 38(1): 120-126. doi: 10.3724/SP.J.1123.2019.01051 shu

Citation: CHEN Zhengyi, LI Yunda, ZHANG Zhuomin, LI Gongke. Rapid determination of 12 semi-volatile pesticide residues in vegetables by miniaturized thermal-assisted purge-and-trap coupled with ultra high pressure liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2020, 38(1): 120-126. doi: 10.3724/SP.J.1123.2019.01051 shu

微热助吹扫捕集-超高压液相色谱-串联质谱法快速测定蔬菜中12种半挥发性农药残留

中山大学化学学院, 广东广州 510275
基金项目:
国家自然科学基金（21675178，21575167，21976213）；广东省公益研究与能力建设专项基金（2015A030401036）；广州市科技计划项目（201604020165）；广东省食品安全重点领域研发计划（2019B020211001）.

摘要: 将微热助吹扫捕集技术与超高压液相色谱-串联质谱（UHPLC-MS/MS）结合，建立了蔬菜中12种半挥发性农药残留的快速分析方法。系统优化了前处理条件，在10 min内即可完成样品前处理过程。12种半挥发性农药残留在各自范围内线性关系良好，相关系数（R²）为0.9918~0.9997；检出限为0.3~1.3 μg/kg，定量限为1.0~4.3 μg/kg。实际样品的加标回收率为80.0%~120.0%，日内和日间精密度均小于10%。该方法具有基质净化效果好、前处理过程简单和分析速度快等优点，可用于蔬菜中多种半挥发农药残留的同时快速分析。

关键词:

English

Rapid determination of 12 semi-volatile pesticide residues in vegetables by miniaturized thermal-assisted purge-and-trap coupled with ultra high pressure liquid chromatography-tandem mass spectrometry

School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China
Received Date: 30 January 2019
Fund Project:
National Natural Science Foundation of China (Nos. 21675178, 21575167)

Abstract: A method for the analysis of 12 semi-volatile pesticide residues in vegetables was developed by miniaturized thermal-assisted purge-and-trap coupled with ultra high pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Under the optimized conditions, the sample preparation could be completed within 10 min. The linear relationships of the 12 semi-volatile pesticide residues were good within their corresponding concentration ranges. The correlation coefficients were in range of 0.9918-0.9997. The limits of detection and limits of quantification were in range of 0.3-1.3 μg/kg and 1.0-4.3 μg/kg, respectively. The recoveries ranged from 80.0% to 120.0%, with intra-day and inter-day RSDs of less than 10%. This proposed method is quick and simple, and is suitable for the simultaneous and rapid analysis of semi-volatile pesticide residues in vegetables.

Key words:

1. [1] Zhang L, Zhang J S, Jiang S Q, et al. Occupation and Health, 2016, 32(4):569 张力, 张静姝, 姜淑卿, 等. 职业与健康, 2016, 32(4):569
2. [2] Lu Z M, Han D D, Meng F X, et al. Medical Research and Education, 2018, 35(1):70 陆治名, 韩丹丹, 孟凡轩, 等. 医学研究与教育, 2018, 35(1):70
3. [3] GB/T 2763-2016
4. [4] Elibariki R, Maguta M M. Chemosphere, 2017, 178:154
5. [5] Raju S M, Bidlan R. Biosci. Biotech Res Comm, 2017, 10(4):697
6. [6] Barganska Z, Lambropoulou D, Namiesnik J. Crit Rev Anal Chem, 2018, 48(6):447
7. [7] Stachniuk A, Fornal E. Food Anal Methods, 2016, 9(6):1654
8. [8] Oellig C. J Chromatogr A, 2016, 1445:19
9. [9] Song Y, Ma R, Hao L, et al. J Chromatogr A, 2018, 1572:20
10. [10] Zhang X Z, Chen Z M, Zhao M Q, et al. Journal of Instrument Analysis, 2018, 37(10):1221 张新忠, 陈宗懋, 赵梅勤, 等. 分析测试学报, 2018, 37(10):1221
11. [11] Lu J F, Liu J Y. Shanghai Vegetables, 2018(5):70 陆佳菲, 刘佳莹. 上海蔬菜, 2018(5):70
12. [12] Wang M, Zhou X, Zang X, et al. J Sep Sci, 2018, 41(21):4038
13. [13] Naccarato A, Pawliszyn J. Food Chem, 2016, 206:67
14. [14] Wang Q.[MS Dissertation]. Taiyuan:Shanxi University, 2017 王祺.[硕士学位论文]. 太原:山西大学, 2017
15. [15] Qiu B, Wang X, Li H, et al. Food Anal Methods, 2018, 11(3):693
16. [16] Farajzadeh M A, Mohebbi A, Feriduni B. Anal Chim Acta, 2016, 920:1
17. [17] Zhang A Z, Wang Q L, Cao L L, et al. Chinese Journal of Chromatography, 2016, 34(2):158 张爱芝, 王全林, 曹丽丽, 等. 色谱, 2016, 34(2):158
18. [18] Meng Z J, Huang Y X, Zhao L M, et al. Chinese Journal of Chromatography, 2018, 10(9):917 孟志娟, 黄云霞, 赵丽敏, 等. 色谱, 2018, 10(9):917
19. [19] Li Y H, Zhou P, Xu Q H, et al. Chinese Journal of Chromatography, 2018, 36(2):136 李樱红, 周萍, 徐权华, 等. 色谱, 2018, 36(2):136
20. [20] He S H, Zhang M S, Li T Y, et al. Environmental Chemistry, 2018, 37(8):1880 何书海, 张鸣珊, 李腾崖, 等. 环境化学, 2018, 37(8):1880
21. [21] Su R, Li X, Liu W, et al. J Agric Food Chem, 2016, 64(7):1627
22. [22] Jastrzembski J A, Sacks G L. Anal Chem, 2016, 88(17):8617
23. [23] Nan J, Wang J, Piao X, et al. Talanta, 2015, 142:64
24. [24] Chienthavorn O, Dararuang K, Sasook A, et al. Anal Bioanal Chem, 2012, 402(2):955
25. [25] Chen Z, Li G, Zhang Z. Anal Chem, 2017, 89(17):9593
26. [26] GB/T 23200.14-2016
27. [27] Deng H F, Zhang J Y, Huang K, et al. Chinese Journal of Chromatography, 2018, 36(12):1211 邓慧芬, 张建莹, 黄科, 等. 色谱, 2018, 36(12):1211
28. [28] Li L Y, Xu X M, Lin H, et al. Chinese Journal of Chromatography, 2016, 34(9):835 李凌云, 许晓敏, 林桓, 等. 色谱, 2016, 34(9):835
29. [29] Zhang A Z, Wang Q L, Cao L L, et al. Chinese Journal of Chromatography, 2016, 34(2):158 张爱芝, 王全林, 曹丽丽, 等. 色谱, 2016, 34(2):158

扫一扫看文章

计量

PDF下载量: 2
文章访问数: 91
HTML全文浏览量: 0

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

微热助吹扫捕集-超高压液相色谱-串联质谱法快速测定蔬菜中12种半挥发性农药残留

English

Rapid determination of 12 semi-volatile pesticide residues in vegetables by miniaturized thermal-assisted purge-and-trap coupled with ultra high pressure liquid chromatography-tandem mass spectrometry

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处