Citation: TANG Jiwang, YUAN Liejiang, XIAO Yong, WANG Xiu, WANG Shuxia. Simultaneous determination of nine artificial sweeteners in food by solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2019, 37(6): 619-625. doi: 10.3724/SP.J.1123.2019.01012 shu

Simultaneous determination of nine artificial sweeteners in food by solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry

1.
Hunan Testing Institute of Product and Commodity Supervison, Changsha 410007, China;
2.
The chemical and chemical engineering academy of central south university, Changsha 410083, China

Received Date: 14 January 2019

Fund Project: Innovation-Driven Plan in Administration of Quality and Technology Supervision of Hunan Province (No. 2017KYJH06).

A simple and rapid method based on solid-phase extraction and high performance liquid chromatography-tandem mass spectrometry for the simultaneous determination of nine artificial sweeteners (acesulfame-K, saccharin sodium, cyclamate, sucralose, aspartame, alitame, neotame, dulcin, neohesperidin dihydrochalcone) in various foods was developed. The sweeteners in food samples were extracted with triethylamine buffer solution (pH 4.5) and cleaned using an SPE column equipped with hydrophilic and lipophilic packing material. The analytes were separated on a Phenomenex Knietex^® F5 column (100 mm×2.1 mm, 2.6 μm) using 0.1% (v/v) formic acid-5 mmol/L ammonium formate/methanol as a mobile phase for gradient elution, and then determined by tandem mass spectrometry in positive and negative ESI modes under multiple reaction monitoring (MRM). The internal standard method was used to further suppress the matrix effect. The method proved to be very effective in the removal of matrix interferences. Calibration curves were linear within a studied range of concentrations (r²>0.999) for the nine artificial sweeteners. The limits of detection (LODs) and limits of quantification (LOQs) were within 2-30 μg/kg and 6-100 μg/kg, respectively. The recoveries for the nine investigated sweeteners were within 86.3%-106.3% at three spiked levels, with relative standard deviations (RSDs) between 1.2% and 5.9%. The developed method is rapid, efficient, accurate, and reliable; it can also be applied for the rapid determination of other artificial sweeteners in a complex food matrix.
- high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- solid-phaseextraction (SPE),
- artificial sweetener,
- food
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
1. [1]
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沈阳化工大学材料科学与工程学院沈阳 110142