Citation: HU Wenwen, TAO Jianwei, WANG Qingwei, LI Jing, YAN Chao, XU Xu. Simultaneous determination of vitamins B₁, B₂, B₆, niacinamide and calcium pantothenate in compound vitamin B tablets by high performance quantitative capillary electrophoresis[J]. Chinese Journal of Chromatography, ;2019, 37(6): 661-665. doi: 10.3724/SP.J.1123.2018.12040 shu

Simultaneous determination of vitamins B₁, B₂, B₆, niacinamide and calcium pantothenate in compound vitamin B tablets by high performance quantitative capillary electrophoresis

HU Wenwen ^1, ,
TAO Jianwei ¹ ,
WANG Qingwei ¹ ,
LI Jing ² ,
YAN Chao ^2,3 ,
XU Xu ¹

1.
School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China;
2.
Unimicro Technologies, Inc., Shanghai 201203, China;
3.
Pharmacy School, Shanghai Jiaotong University, Shanghai 200240, China

Received Date: 28 December 2018

A high performance quantitative capillary electrophoresis method was developed for the simultaneous determination of vitamins B₁, B₂, B₆, nicotinamide, and calcium pantothenate in vitamin B tablets using a quantitative capillary electrophoresis instrument. The samples were extracted ultrasonically with acetonitrile-water (20:80, v/v). The automatic high precision quantitative capillary electrophoresis instrument was used to realize quantitative injection through a 10 nL injection valve. The background electrolyte was selected as 40 mmol/L sodium borate buffer (pH 9.0), which was continuously supplied by a microfluidic injection pump. The working voltage was -10 kV. The detection wavelength of vitamins B₁, B₂, B₆, and nicotinamide was selected as 280 nm, which was then changed to 210 nm to detect calcium pantothenate. The result showed good linearities between the peak area and the concentration of vitamins B₁, B₂, B₆, nicotinamide, and calcium pantothenate in the correlation coefficients (r) range of 0.9968-0.9998. The limits of detection (LODs) were in the range of 2.5-36.0 mg/L. The average recoveries were 94.1%-98.9% with relative standard deviations (RSDs) as 1.3%-1.9%. The method is precise, reliable, and suitable for the simultaneous determination of vitamins B₁, B₂, B₆, nicotinamide, and calcium pantothenate in a real compound vitamin B tablet.
- capillary electrophoresis (CE),
- compound vitamin B tablets,
- content determination
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沈阳化工大学材料科学与工程学院沈阳 110142

Simultaneous determination of vitamins B1, B2, B6, niacinamide and calcium pantothenate in compound vitamin B tablets by high performance quantitative capillary electrophoresis

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通讯作者: 陈斌, bchen63@163.com

Simultaneous determination of vitamins B₁, B₂, B₆, niacinamide and calcium pantothenate in compound vitamin B tablets by high performance quantitative capillary electrophoresis