中空纤维膜液相微萃取-超高效液相色谱测定蒙药毛勒日-达布斯-4汤中两种生物碱

引用本文: 宝贵荣, 孟和, 李优鑫, 包建民. 中空纤维膜液相微萃取-超高效液相色谱测定蒙药毛勒日-达布斯-4汤中两种生物碱[J]. 色谱, 2019, 37(6): 644-648. doi: 10.3724/SP.J.1123.2018.12029 shu

Citation: BAO Guirong, MENG He, LI Youxin, BAO James Jianmin. Determination of two alkaloids in Mongolian medicine Mauleri-Dabusi-4 soup by hollow fiber liquid phase micro-extraction coupled with ultra-high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2019, 37(6): 644-648. doi: 10.3724/SP.J.1123.2018.12029 shu

中空纤维膜液相微萃取-超高效液相色谱测定蒙药毛勒日-达布斯-4汤中两种生物碱

1.
呼和浩特职业学院生物化学工程学院, 内蒙古呼和浩特 010051;
2.
天津大学药物科学与技术学院, 天津 300072;
3.
内蒙古大学化学化工学院, 内蒙古呼和浩特 010021
基金项目:
内蒙古自然科学基金项目（2018MS02007）.

摘要: 建立了将中空纤维膜液相微萃取（HF-LPME）技术与超高效液相色谱（UPLC）技术联用检测蒙药毛勒日-达布斯-4汤中2种生物碱（胡椒碱和荜茇宁）的分析方法。通过考察该HF-LPME方法的影响参数，优化了萃取实验条件。HF-LPME优化条件如下：空隙率大于50%的偏氟乙烯中空纤维膜，萃取溶剂为正辛醇，氯化钠质量浓度为10 g/L，室温振荡，振荡速度为173 r/min，萃取时间为128 min。结果表明：该HF-LPME-UPLC方法对胡椒碱和荜菝宁的检出限（LOD）分别为2.2和2.5 μg/L，相对标准偏差不大于7.8%（n=5）。胡椒碱和荜菝宁分别在100~8500 和8.3~5000 μg/L范围内具有良好的线性关系，胡椒碱和荜茇宁的富集倍数分别为59和65。该方法简便、快速、准确、环保，适用于蒙药中胡椒碱和荜菝宁含量的测定。

关键词:

English

Determination of two alkaloids in Mongolian medicine Mauleri-Dabusi-4 soup by hollow fiber liquid phase micro-extraction coupled with ultra-high performance liquid chromatography

1.
Department of Biochemistry Engineering, Hohhot Vocational College, Hohhot 010051, China;
2.
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China;
3.
College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, China
Received Date: 20 December 2018
Fund Project:
Inner Mongolia Natural Science Foundation (No. 2018MS02007).

Abstract: In this study, a method combining hollow fiber liquid phase micro-extraction (HF-LPME) and ultra-high performance liquid chromatography (UPLC) was developed for the determination of two alkaloids (piperine and piperlonguminine) in Mongolian medicine Mauleri-Dabusi-4 Soup. The parameters affecting the micro-extraction efficiency were evaluated and optimized. The optimum conditions were as follows:polyvinylidence fluoride HF (54% pore size); 10 g/L NaCl; 30 μ L octanol as extraction solvent; 173 r/min stirring rate of extraction; and 128 min extraction time. The extracted drug was detected by UPLC. The calibration curves obtained good linear relationship in the concentration ranges of 100-8500 and 8.3-5000 μg/L for piperine and piperlonguminine, respectively. The enrichment factors of the method for piperine and piperlonguminine were achieved to be 59 and 65. The limits of detection were 2.2 μg/L for piperine and 2.5 μg/L for piperlonguminine. The method was successfully applied for the determination of alkaloids in Mongolian medicine Mauleri-Dabusi-4.

Key words:

hollow fiber liquid phase micro-extraction (HF-LPME)
/ ultra-high performance liquid chromatography (UPLC)
/ alkaloids
/ Mauleri-Dabusi-4
/ Mongolian medicine

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中空纤维膜液相微萃取-超高效液相色谱测定蒙药毛勒日-达布斯-4汤中两种生物碱

English

Determination of two alkaloids in Mongolian medicine Mauleri-Dabusi-4 soup by hollow fiber liquid phase micro-extraction coupled with ultra-high performance liquid chromatography

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中国化学会秘书处

中空纤维膜液相微萃取-超高效液相色谱测定蒙药毛勒日-达布斯-4汤中两种生物碱

English

Determination of two alkaloids in Mongolian medicine Mauleri-Dabusi-4 soup by hollow fiber liquid phase micro-extraction coupled with ultra-high performance liquid chromatography

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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