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Citation: BAO Guirong, MENG He, LI Youxin, BAO James Jianmin. Determination of two alkaloids in Mongolian medicine Mauleri-Dabusi-4 soup by hollow fiber liquid phase micro-extraction coupled with ultra-high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2019, 37(6): 644-648. doi: 10.3724/SP.J.1123.2018.12029 shu

Determination of two alkaloids in Mongolian medicine Mauleri-Dabusi-4 soup by hollow fiber liquid phase micro-extraction coupled with ultra-high performance liquid chromatography

1.
Department of Biochemistry Engineering, Hohhot Vocational College, Hohhot 010051, China;
2.
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China;
3.
College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, China

Received Date: 20 December 2018

Fund Project: Inner Mongolia Natural Science Foundation (No. 2018MS02007).

In this study, a method combining hollow fiber liquid phase micro-extraction (HF-LPME) and ultra-high performance liquid chromatography (UPLC) was developed for the determination of two alkaloids (piperine and piperlonguminine) in Mongolian medicine Mauleri-Dabusi-4 Soup. The parameters affecting the micro-extraction efficiency were evaluated and optimized. The optimum conditions were as follows:polyvinylidence fluoride HF (54% pore size); 10 g/L NaCl; 30 μ L octanol as extraction solvent; 173 r/min stirring rate of extraction; and 128 min extraction time. The extracted drug was detected by UPLC. The calibration curves obtained good linear relationship in the concentration ranges of 100-8500 and 8.3-5000 μg/L for piperine and piperlonguminine, respectively. The enrichment factors of the method for piperine and piperlonguminine were achieved to be 59 and 65. The limits of detection were 2.2 μg/L for piperine and 2.5 μg/L for piperlonguminine. The method was successfully applied for the determination of alkaloids in Mongolian medicine Mauleri-Dabusi-4.
- hollow fiber liquid phase micro-extraction (HF-LPME),
- ultra-high performance liquid chromatography (UPLC),
- alkaloids,
- Mauleri-Dabusi-4,
- Mongolian medicine
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