Citation: WANG Siwei, ZENG Guangfeng, LIU Yanping, WANG Xiaonan, SUN Haibin. Determination of glufosinate-ammonium and its three metabolites in litchi and banana by high performance liquid chromatography coupled with triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2019, 37(1): 93-99. doi: 10.3724/SP.J.1123.2018.08002 shu

Determination of glufosinate-ammonium and its three metabolites in litchi and banana by high performance liquid chromatography coupled with triple quadrupole mass spectrometry

1.
Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2.
Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China

Received Date: 2 August 2018

Fund Project: National Key Research and Development Plan Project (No. 2016YFD0200206-Y)

A method was developed for the determination of glufosinate-ammonium and its metabolites in litchi and banana by high performance liquid chromatography coupled with triple quadrupole mass spectrometry. The samples were ultrasound extracted by water, centrifuged to precipitate, and filtered through a 0.22 μm Millipore membrane. The sample extract was separated by an Extend-C18 column with 0.1% ammonia-methanol (9:1, v/v) as mobile phase with an isocratic elution, identified by electrospray ionization in negative and multiple reaction monitoring modes, and quantified with external standard method. Moreover, the pretreatment and mass spectrometry conditions were discussed. The method showed good linear relationship in the range of 1-1000 μg/L for glufosinate-ammonium and its metabolites. The average recoveries ranged from 82.9% to 98.6% with the relative standard deviations of 2.6%-6.3% when the litchi and banana samples were spiked at 50, 100, 1000 μg/kg. The limits of detection were 5-10 μg/kg. The method has rapid analysis speed as well as high reliability and sensitivity, which are suitable for the fast determination of glufosinate-ammonium and its metabolites in litchi and banana samples.
- high performance liquid chromatography (HPLC),
- triple quadrupole mass spectrometry (MS/MS),
- glufosinate-ammonium,
- metabolites,
- litchi,
- banana
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沈阳化工大学材料科学与工程学院沈阳 110142