Citation: ZHANG Kai, QIN Yu, BIAN Hua, CAO Hui, LIU Tianyi, GE Yu. Determination of eight antifungal drugs in mutton by QuEChERS coupled with ultra-performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2018, 36(10): 999-1004. doi: 10.3724/SP.J.1123.2018.05028 shu

Determination of eight antifungal drugs in mutton by QuEChERS coupled with ultra-performance liquid chromatography-tandem mass spectrometry

ZHANG Kai ^1,2, ,
QIN Yu ² ,
BIAN Hua ² ,
CAO Hui ¹ ,
LIU Tianyi ² ,
GE Yu ²

1.
College of Medical Equipment and Food, University of Shanghai for Science and Technology, Shanghai 200093, China;
2.
Shanghai Institute of Quality Inspection and Technical Research, Shanghai 200233, China

Received Date: 31 May 2018

Fund Project: Shanghai Science and Technology Prosperous Agriculture Key Project (Chinese Characters of Shanghai Agricultural Sciences (2016) No. 4-5)

A method for the simultaneous determination of eight antifungal drugs in mutton was developed using QuEChERS combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were shaken with acetonitrile containing 2.0% (v/v) formic acid, and then subjected to QuEChERS purification. The residual targets were analyzed on an Atiantis^® T3 column (100 mm×2.1 mm, 3 μm) with gradient elution using acetonitrile-0.1% (v/v) formic acid aqueous solution as the mobile phase. All the analytes were effectively separated and cleaned up within 9 min, and were finally detected by UPLC-MS/MS with ESI⁺ ionization in multiple reaction monitoring (MRM) mode. All the eight antifungal drugs showed a good linear trend in the range of 0.2-50 μg/L, with the correlation coefficient (R²) greater than 0.99. The limits of detection (LODs) and limits of quantification (LOQs) were 0.3-3.0 μg/kg and 1.0-10.0 μg/kg, respectively. The calibration curves were used in the analysis to reduce the negative influence of matrix effects. The average recoveries at different levels (low, medium, and high) for all the analytes ranged from 70.3% to 118.4%, with the relative standard deviations of 0.4%-4.3% (n=6). The developed method shows excellent recovery and stable repeatability, and is suitable for the determination of the antifungal drugs in mutton.
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- QuEChERS,
- antifungal drugs,
- mutton
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