Citation: YANG Zhi, PENG Yan, JIN Mengna, LIN Liqin. Simultaneous rapid determination of 12 anti-allergic chemical drugs in Chinese traditional patent medicine and health food by supercritical fluid chromatography tandem mass spectrometry with solid phase extraction[J]. Chinese Journal of Chromatography, ;2018, 36(9): 889-894. doi: 10.3724/SP.J.1123.2018.04006 shu

Simultaneous rapid determination of 12 anti-allergic chemical drugs in Chinese traditional patent medicine and health food by supercritical fluid chromatography tandem mass spectrometry with solid phase extraction

Hangzhou Institute for Food and Drug Control, Hangzhou 310022, China

Received Date: 4 April 2018

Fund Project: Science and Technology Project of Zhejiang Food and Drug Administration (No. BH201601).

An analytical method was developed for simultaneous rapid determination of 12 anti-allergic chemical drugs in Chinese traditional patent medicine and health food by supercritical fluid chromatography tandem mass spectrometry with solid phase extraction (SPE-SFC-MS/MS). Samples were extracted with methanol by sonification and then purified by Oasis mixed-model cation exchange SPE. The extracts were separated on a Waters Trefoil CEL1 (150 mm×3.0 mm, 2.5 μ m) column with a mobile phase consisting of carbon dioxide-methanol containing 0.1% (v/v) ammonia water in a gradient elution mode, at a flow rate of 1.2 mL/min. The column temperature was 45℃ and the back pressure was 12.4×10⁶ Pa. The whole analysis was completed in 10 min. The 12 anti-allergic chemical drugs were detected by an electrospray ion source in positive or negative modes with a multiple reaction monitoring (MRM) mode. The calibration curves of the 12 anti-allergic chemical drugs showed good linearities in the range of 5-250 μ g/L with the correlation coefficients (r) ≥ 0.998. The limits of detection (LODs) were 0.141-0.262 μ g/L, and the limits of quantification (LOQs) were 0.703-1.308 μ g/L. The recoveries of the 12 anti-allergic chemical drugs at spiked levels of 10, 20 and 100 μ g/L were in the range of 76.1%-112.5%, and the relative standard deviations (RSDs) were 1.1%-8.3% (n=6). The method is simple, sensitive and reliable. It has been successfully used for the detection of illegally added anti-allergic chemical drugs in Chinese traditional patent medicine and health food.
- supercritical fluid chromatography (SFC),
- mass spectrometry (MS),
- solid phase extraction (SPE),
- anti-allergic chemical drug,
- Chinese traditional patent medicine,
- health food
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