Citation: CHEN Hongxu, QU Feng. Applications on therapeutic monoclonal antibody analysis by capillary electrophoresis[J]. Chinese Journal of Chromatography, ;2018, 36(3): 195-208. doi: 10.3724/SP.J.1123.2018.01015 shu

Applications on therapeutic monoclonal antibody analysis by capillary electrophoresis

CHEN Hongxu ,
QU Feng

School of Life Science, Beijing Institute of Technology, Beijing 100081, China

Received Date: 9 January 2018

Fund Project: National Natural Science Foundation of China (Nos. 21675012, 21375008).

Therapeutic monoclonal antibodies play an important role in biopharmaceuticals, and gradually become one of the main directions of the development of biological medicine. The quality control of monoclonal antibodies has also become the focus of global biopharma companies and regulatory agencies. Capillary electrophoresis has become an important tool in the analysis of monoclonal antibodies with multi-modes and high resolution. In this paper, we reviewed the application of capillary gel electrophoresis, capillary isoelectric focusing and capillary zone electrophoresis on the analysis of purity, isoelectric point/charge heterogeneity and N-glycan profiling of therapeutic monoclonal antibodies.
- capillary electrophoresis (CE),
- capillary gel electrophoresis (CGE),
- capillary zone electrophoresis (CZE),
- capillary isoelectric focusing (CIEF),
- therapeutic monoclonal antibody (mAb),
- purity,
- charge heterogeneity,
- N-glycan,
- review
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