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Citation: ZHANG Wenjuan, SONG Naizhong, JIA Qiong. Preparation of cobalt phthalocyanine functionalized polymer monolith and application to enrichment of transferrin glycopeptides[J]. Chinese Journal of Chromatography, ;2018, 36(3): 185-189. doi: 10.3724/SP.J.1123.2017.11045 shu

Preparation of cobalt phthalocyanine functionalized polymer monolith and application to enrichment of transferrin glycopeptides

College of Chemistry, Jilin University, Changchun 130012, China

Received Date: 24 November 2017

Fund Project: Open Project of State Key Laboratory (No. sklssm201719).

Poly(glycidyl methacrylate-ethyleneglycol dimethacrylate) (Poly(GMA-EDMA)) monolith functionalized with cobalt phthalocyanine tetracarboxylic acid (CoPcTc) was prepared. The monolith was used for transferrin (Tf) glycopeptide enrichment. By taking advantage of hydrogen bonds between isoindole subunits of phthalocyanine and glycans and coordination interaction between cobalt and glycopeptides, the monolithic material was efficient and selective. After enrichment of transferrin through the functionalized monolith, 17 glycopeptides were identified by electrospray ionization quadrupole time-of-flight mass spectrometry. When the concentration of transferrin was reduced to 8.8×10^-10mol/L, three glycopeptides could still be obtained. The present method has great potential for trace sample analysis.
- preparation,
- polymer monolith,
- enrichment,
- phthalocyanine,
- transferrin (Tf)
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