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Citation: XIAO Han, Kevin YAN, ZHENG Yiting, WANG Yan, YAN Chao. Study on effect of ionic strength on photolysis of vitamin B₂ based on pressurized capillary electrochromatography coupled with laser induced fluorescence detection[J]. Chinese Journal of Chromatography, ;2018, 36(4): 381-387. doi: 10.3724/SP.J.1123.2017.11014 shu

Study on effect of ionic strength on photolysis of vitamin B₂ based on pressurized capillary electrochromatography coupled with laser induced fluorescence detection

XIAO Han ^1, ,
Kevin YAN ² ,
ZHENG Yiting ¹ ,
WANG Yan ¹ ,
YAN Chao ¹

1.
School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China;
2.
University of California, Riverside 92521, USA

Received Date: 9 November 2017

Fund Project: Shanghai Science and Technology Commission Scientific Research Project (Nos. 16142200300, 17142201000)

A new method for the separation and analysis of vitamin B₂ and its fluorescent photolysis products was established based on pressurized capillary electrochromatography (pCEC) coupled with laser induced fluorescence (LIF) detection.The relationship between the photolysis reaction rate of vitamin B₂ and the ionic strength of aqueous solution or phosphate buffered solution was studied.The analysis was carried out by using a C18 capillary column.The mobile phase was acetonitrile-water with 0.1%(v/v) trifluoroacetic acid with gradient elution.The excitation wavelength was 488 nm and the emission wavelength was 520 nm.Vitamin B₂ and many fluorescent photolysis products were well separated.Based on the above, the influence of photo degradation rate of vitamin B₂ with ionic strength was studied.The apparent rate constant of photolysis was calculated by kinetic calculation.The results showed that the ionic strength had a significant effect on the photolysis of vitamin B₂ solution.The photolysis rate of vitamin B₂ was positively related to the ionic strength.This study provides a highly efficient method for the stability research of vitamin B₂, which may serve as a good reference for the preservation and clinical use of it.
- pressurized capillary electrochromatography (pCEC),
- laser induced fluorescence detection (LIF),
- vitamin B₂,
- photolysis reaction,
- reaction rate constant
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Study on effect of ionic strength on photolysis of vitamin B2 based on pressurized capillary electrochromatography coupled with laser induced fluorescence detection

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Study on effect of ionic strength on photolysis of vitamin B₂ based on pressurized capillary electrochromatography coupled with laser induced fluorescence detection