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Citation: SHEN Fei, XU Yanjuan, JIANG Sheng, ZHAO Bin, SHI Junzhe, CHEN Jing. Determination of microcystins in algal bloom samples by ultra performance liquid chromatography-tandem mass spectrometry and elimination of their matrix effects[J]. Chinese Journal of Chromatography, ;2017, 35(7): 772-777. doi: 10.3724/SP.J.1123.2017.03046 shu

Determination of microcystins in algal bloom samples by ultra performance liquid chromatography-tandem mass spectrometry and elimination of their matrix effects

SHEN Fei ^1,2, ,
XU Yanjuan ¹ ,
JIANG Sheng ³ ,
ZHAO Bin ¹ ,
SHI Junzhe ¹ ,
CHEN Jing ¹

1.
Wuxi Environmental Monitoring Central Station, Wuxi 214121, China;
2.
School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China;
3.
Jiangsu Environmental Monitoring Center, Nanjing 210036, China

Received Date: 31 March 2017

Fund Project: Environmental Monitoring and Research Fund of Jiangsu Province (No.1621)

A rapid and simple method was commonly used for the determination of microcystins by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The reliability, accuracy and sensitivity of LC-MS/MS determination were affected by the matrix effect because of the complex matrices in algal bloom samples. In this study, the matrix interference and matrix effect problems were effectively reduced or eliminated by diluting samples, optimizing gradient elution programs, and reducing injection volumes. The correlation coefficients (r²) of the seven microcystins were ≥ 0.99. The limits of detection and limits of quantification ranged from 0.064 μ g/L to 0.103 μ g/L and 0.213 μ g/L to 0.343 μ g/L, respectively. Algal bloom samples were determined by diluting 10 times and injecting 5 μ L of sample volume. The method provides some references for the determination of microcystins. It is of great significance for ensuring the safety of drinking water source.
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- matrix effect,
- microcystins,
- algal bloom samples
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