Citation: ZHAO Jiaying, LI Xiaomin, LU Xiaohua, ZHANG Qinghe, ZHANG Weibing. Determination of four polycyclic aromatic hydrocarbons in olive oil by high performance liquid chromatography-fluorescent detection[J]. Chinese Journal of Chromatography, ;2017, 35(7): 748-754. doi: 10.3724/SP.J.1123.2017.03040 shu

Determination of four polycyclic aromatic hydrocarbons in olive oil by high performance liquid chromatography-fluorescent detection

1.
College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China;
2.
Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China

Received Date: 29 March 2017

Fund Project: National Key Research and Development Program of China (No.2016YFF0201106).

A method for the determination of four polycyclic aromatic hydrocarbons (PAHs) such as benzo[a]anthracene, chrysene, benzo[b]fluoranthene and benzo[a]pyrene in olive oil by high performance liquid chromatography-fluorescent detection (HPLC-FLD) was developed. The olive oil samples were diluted with isopropanol and cleaned-up by a solid phase extraction cartridge with π-π specificity. Critical isomers were separated by gradient elution with water-acetonitrile on an Agilent ZORBAX Eclipse PAH column (100 mm×2.1 mm, 1.8 μ m). The calibration was performed by the use of matrix-matched calibration standards. The results showed that there were good linear relationships for the four PAHs in the range of 2.4-40 μ g/L with correlation coefficients of 0.9990-0.9999. The limits of quantification (LOQs) ranged from 0.147 to 0.413 μ g/L, and the recoveries of the spiked samples ranged from 95.5% to 103.2%. The intra-day and inter-day precisions were in the ranges of 0.10%-1.69% and 2.48%-2.93% (n=5), respectively. The method has the characteristics of high sensitivity, low detection limit and good precision, and is suitable for rapid and accurate quantitative detection of the four PAHs in olive oil.
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1.
沈阳化工大学材料科学与工程学院沈阳 110142