Citation: WANG Hui, LIU Jiang, LI Xihui, CAO Yang, HUANG Xiaobei, LI Yongqiang. Determination of choline chloride in milk powder and jelly by solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2017, 35(5): 558-562. doi: 10.3724/SP.J.1123.2016.12024 shu

Determination of choline chloride in milk powder and jelly by solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry

Changsha Institute for Food and Drug Control, Food Safety Production Engineering Research Center of Hunan Province, Changsha 410013, China

Received Date: 12 December 2016

Fund Project: Key Program of Hunan Provincial Science and Technology Department (No. 2014SK2011).

An analytical method was developed for the determination of choline chloride in milk powder and jelly by solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were centrifuged after hydrolyzation 3 h in 10 mL 0.02 mol/L ammonium acetate (glacial acetic acid adjust pH to 3.0). The supernatant was cleaned up by a DIKMA ProElut PLS column (60 mg/3 mL). The target compounds were separated by an Agilent ZORBAX 300 SCX column (150 mm×2.1 mm, 5 μm). Qualitative and quantitative analyses were carried out by multiple reaction monitoring (MRM) mode with positive electrospray ionization (ESI⁺). The limit of detection (LOD, S/N=3) and the limit of quantitation (LOQ, S/N=10) were 0.15 mg/kg and 0.50 mg/kg, respectively. The recoveries of choline chloride spiked in milk powder and jelly were 70.8%-100.2%, with RSDs no more than 6.83% (n=6). The standard curves showed good linearity in the concentration range from 0.05 to 8.0 mg/L with correlation coefficient (r) of 0.996. The method was applied to detection of commercially available choline chloride in milk powder and jelly. The contents of choline chloride in real milk power and jelly samples were 251.0-2448 mg/kg and 0.261-0.314 mg/kg, respectively. The developed method is accurate, reliable, sensitive, and suitable for the determination of choline chloride in milk powder and jelly.
- ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- solid phase extraction (SPE),
- choline chloride,
- milk powder,
- jelly
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