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Citation: ZHAO Haijie, LIANG Wenhui, LI Zhen, SHI Qinghao, JIAN Fangfang, FA Yun, LIU Huizhou. Capillary ion chromatography-mass spectrometry for determination of low relative molecular mass organic acids in culture media of Clostridium thermocellum[J]. Chinese Journal of Chromatography, ;2017, 35(3): 264-268. doi: 10.3724/SP.J.1123.2016.10010 shu

Capillary ion chromatography-mass spectrometry for determination of low relative molecular mass organic acids in culture media of Clostridium thermocellum

ZHAO Haijie ^1,2, ,
LIANG Wenhui ² ,
LI Zhen ³ ,
SHI Qinghao ^1,2 ,
JIAN Fangfang ¹ ,
FA Yun ^2,4 ,
LIU Huizhou ²

1.
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;
2.
Key Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China;
3.
Qingdao YIKESI Technology Engineering Company Limited, Qingdao 266045, China;
4.
University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 8 October 2016

Fund Project: National Natural Science Foundation of China (No. 21405166)

A capillary ion chromatography-mass spectrometry method was proposed to determine low relative molecular mass organic acids in culture media of Clostridium thermocellum. The conditions of analysis were optimized and eight organic acids were detected simultaneously. The target compounds were separated on an IonSwift MAX-100 capillary column with potassium hydroxide solution as mobile phase by gradient elution, and detected under selected ion monitoring (SIM) mode. The mass spectrometry conditions were: spray voltage 3.0 kV, sheath gas 2000 kPa, capillary temperature 275℃. The relative standard deviations ranged from 1.45% to 5.99%. The coefficients of determination ranged from 0.9696 to 0.9986 and the mean recoveries were 89.0%-110.0%. The limits of detection of the eight organic acids were 0.01-0.50mg/L. This method was used to detect low relative molecular mass organic acids in culture media of Clostridium thermocellum with high sensitivity and good reproducibility.
- capillary ion chromatography,
- mass spectrometry (MS),
- organic acids,
- Clostridium thermocellum
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