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Citation: JIA Xiangyang, YOU Huiyan, FU Xiuli. Fast detection of kanamycin in milk by protamine-aptamer-gold nanotechnology[J]. Chinese Journal of Chromatography, ;2017, 35(3): 269-273. doi: 10.3724/SP.J.1123.2016.10007 shu

Fast detection of kanamycin in milk by protamine-aptamer-gold nanotechnology

1.
College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China;
2.
Department of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China;
3.
Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China

Received Date: 8 October 2016

Fund Project: National Key Scientific Instrument and Equipment Development Project (No. 2013YQ17052506)

A bio-nanodetection system was developed for kanamycin based on electrostatic interaction between polycationic protamine and negatively charged aptamer or gold nanoparticles (AuNPs). The concentrations of protamine, aptamer and cations in the buffer solution were optimized. The results showed that there was a good linear correlation (R²=0.9928) between gold nanoparticles and kanamycin in the range of 5-5000 nmol/L with 20 mmol/L Na⁺, 1 mmol/L Mg²⁺, 2 mg/L protamine and 100 nmol/L aptamer. The limit of detection was 0.53 nmol/L. Under this experimental condition, the content of kanamycin in milk was detected, the recoveries were 96%-98%, and the relative standard deviations (RSDs) were 1.5%-3.2%. This method has the advantages of high selectivity, good sensitivity and wide linear range, and is useful in the detection of kanamycin in foods.
- protamine,
- aptamer,
- gold nanoparticles,
- bio-nano,
- kanamycin,
- milk
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