一种新型核壳型色谱固定相在高效液相色谱中的评价及应用

引用本文: 孙元社, 瞿其曙, 于淑新, 李霜霜, 唐涛, 李彤. 一种新型核壳型色谱固定相在高效液相色谱中的评价及应用[J]. 色谱, 2016, 34(12): 1250-1254. doi: 10.3724/SP.J.1123.2016.09006 shu

Citation: SUN Yuanshe, QU Qishu, YU Shuxin, LI Shuangshuang, TANG Tao, LI Tong. Evaluation and application of a new core-shell chromatographic stationary phase for high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2016, 34(12): 1250-1254. doi: 10.3724/SP.J.1123.2016.09006 shu

一种新型核壳型色谱固定相在高效液相色谱中的评价及应用

1.
大连依利特分析仪器有限公司, 辽宁大连 116023;
2.
辽宁大连依利特分析仪器工程技术研究中心, 辽宁大连 116023;
3.
安徽建筑大学材料与化学工程学院, 安徽合肥 230601
基金项目:
国家重大科学仪器设备开发专项（2012YQ12004403）；国家自然科学基金（51472121）；大连市科技计划项目（2015A11GX028）.

摘要: 采用扫描电镜、透射电镜和氮气吸附法对制备的新型核壳型色谱固定相进行了表征，结果表明该固定相单分散性好、表面放射状孔道壳层结构均一、孔径分布窄。对该核壳材料的表面进行C18键合修饰，考察其基本色谱性能，色谱柱的理论塔板数超过150000块/m，色谱峰峰形对称，甲苯与乙苯的保留因子之比为1.45，亚甲基选择性优异。将该核壳材料应用于汽车尾气中醛酮类化合物的检测，在优化的色谱条件下，2，4-二硝基苯肼（DNPH）衍生的醛酮类化合物在15 min内获得了较好的分离效果。该核壳型C18色谱固定相具有分离速度快、选择性好、柱效高等特点，适于复杂样品的高效、快速分离分析。

关键词:

English

Evaluation and application of a new core-shell chromatographic stationary phase for high performance liquid chromatography

1.
Dalian Elite Analytical Instruments Co., Ltd., Dalian 116023, China;
2.
Liaoning Engineering Research Center of Dalian Elite Analytical Instruments, Dalian 116023, China;
3.
School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, China
Received Date: 01 September 2016
Fund Project:
National Major Scientific Instrument and Equipment Development Project (No. 2012YQ12004403)

Abstract: A new core-shell chromatographic stationary phase was characterized by scanning electron microscope, transmission electron microscope and nitrogen adsorption method. The results showed that it had excellent monodispersity, homogeneous structure and narrow pore size distribution. It was modified by C18, and the chromatographic performance was also performed. The number of theoretical plates was over 150000 plates/m, and the chromatographic peaks were symmetrical. The retention factor ratio of toluene and ethylbenzene was 1.45, and the methylene selectivity was excellent. Finally, it was applied to detect aldehydes and ketones in automobile exhaust. The aldehydes and ketones which derivatized by 2,4-dinitrophenylhydrazine (DNPH) were well separated in less than 15 min under the optimized chromatographic conditions. The high separation speed, good selectivity and perfect column efficiency are obtained with the novel core-shell chromatographic stationary.

Key words:

high performance liquid chromatography (HPLC)
/ core-shell chromatographic stationary phase
/ evaluation and application
/ carbonyl compounds

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

一种新型核壳型色谱固定相在高效液相色谱中的评价及应用

English

Evaluation and application of a new core-shell chromatographic stationary phase for high performance liquid chromatography

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

一种新型核壳型色谱固定相在高效液相色谱中的评价及应用

English

Evaluation and application of a new core-shell chromatographic stationary phase for high performance liquid chromatography

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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