Citation: ZHANG Ning, ZHANG Yuan, ZHANG Weibing. Identification and analysis of acetaldehyde induced deoxyribonucleic acid adducts by ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(8): 757-761. doi: 10.3724/SP.J.1123.2016.05025 shu

Identification and analysis of acetaldehyde induced deoxyribonucleic acid adducts by ultra performance liquid chromatography-tandem mass spectrometry

1.
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China

Corresponding author: ZHANG Weibing,
Received Date: 26 May 2016

Fund Project:

Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used for the identification and analysis of the two diastereomers of adducts ((6S, 8S)1, N²-propano-2'-deoxyguanosine (ProdG) and (6R, 8R)ProdG). By contrasting the chromatographic retention time and mass spectrographic fragmentation patterns with ProdG standard, it was proved that ProdG addcuts can be formed from the reaction of 2'-deoxyguanosine (dG) with acetaldehyde. Vitro experiments showed that ProdG adducts can be formed in double stranded deoxyribonucleic acid (DNA) by the induction of acetaldehyde, and the formation of (6R, 8R)ProdG was higher than that of (6S, 8S)ProdG. In cell experiments, acetaldehyde exposure can significantly increase the levels of ProdG adducts in genomic DNA of human embryonic lung fibroblast MRC5 cells, and the enhancement of ProdG was positively correlated with the concentration of acetaldehyde. In addition, the up-regulation of (6R, 8R)ProdG was from 6.4±0.3 to 127.2±2.7 adducts per 10⁸ nucleotides, higher than that of (6S, 8S)ProdG from 6.5±0.3 to 115.3±2.5 adducts per 10⁸ nucleotides by acetaldehyde exposure at 100 μ mol/L. This work provides an experimental basis for the up-regulation of DNA adducts induced by acetaldehyde exposure.
1. [1]
  [1] Wang M Y, Yu N X, Chen L, et al. Chem Res Toxicol, 2006, 19(2): 319
2. [2]
  [2] Wang M, McIntee E J, Cheng G, et al. Chem Res Toxicol, 2000, 13(11): 1149
3. [3]
  [3] Inagaki S, Esaka Y, Deyashiki Y, et al. J Chromatogr A, 2003, 987: 341
4. [4]
  [4] Brooks P J, Zakhari S. Environ Mol Mutagen, 2014, 55(2): 77
5. [5]
  [5] Terashima I, Matsuda T, Fang T W, et al. Biochem, 2001, 40(13): 4106
6. [6]
  [6] Garcia C C, Angeli J P, Freitas F P, et al. J Am Chem Soc, 2011, 133(24): 9140
7. [7]
  [7] Stein S, Lao Y, Yang I Y, et al. Mutat Res, 2006, 608(1): 1
8. [8]
  [8] Wu Y P, Guo L H. Progress in Chemistry, 2014, 26(1): 1 吴一萍, 郭良宏. 化学进展, 2014, 26(1): 1
9. [9]
  [9] Liu B X. Journal of East China University of Science and Technology, 2011, 37(3): 311 刘百祥. 华东理工大学学报, 2011, 37(3): 311
10. [10]
  [10] Feng F, Wang C, Lü M L, et al. Progress in Chemistry, 2009, 21(2/3): 503 冯峰, 王超, 吕美玲, 等. 化学进展, 2009, 21(2/3): 503
11. [11]
  [11] Wang C, Li T, Wang Z, et al. J Chromatogr A, 2010, 1217: 2254
12. [12]
  [12] Wang X, Suo Y, Yin R, et al. J Chromatogr B, 2011, 879: 1647
13. [13]
  [13] Yin R, Liu S, Zhao C, et al. Anal Chem, 2013, 85(6): 3190
14. [14]
  [14] Zhang N, Song Y, Wu D, et al. J Chromatogr A, 2016, 1450: 38
15. [15]
  [15] Zhang N, Song Y, Zhang W, et al. J Chromatogr B, 2016, 1023: 68
16. [16]
  [16] Hinz J M, Czaja W. DNA Repair, 2015, 36: 91
17. [17]
  [17] Caglayan M, Wilson S H. DNA Repair, 2015, 35: 85
18. [18]
  [18] Dizdaroglu M. Mutat Res, 2005, 591(1/2): 45
1. [1]
  Lijun Yue , Siya Liu , Peng Liu . 不同晶相纳米MnO₂的制备及其对生物乙醇选择性氧化催化性能的测试——一个科研转化的综合化学实验. University Chemistry, 2025, 40(8): 225-232. doi: 10.12461/PKU.DXHX202410005
2. [2]
  Cuiping HE , Zhuxuan LI , Yuqing SUN , Jie LIU , Shicheng XU , Zhanchao WU . Ca²⁺ doping induced crystal phase transition and spectral regulation of Ba₃P₄O₁₃: Eu²⁺ phosphor. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2299-2306. doi: 10.11862/CJIC.20250074
3. [3]
  Yajuan Xing , Hui Xue , Jing Sun , Niankun Guo , Tianshan Song , Jiawen Sun , Yi-Ru Hao , Qin Wang . Cu₃P-Induced Charge-Oriented Transfer and Surface Reconstruction of Ni₂P to Achieve Efficient Oxygen Evolution Activity. Acta Physico-Chimica Sinica, 2024, 40(3): 2304046-0. doi: 10.3866/PKU.WHXB202304046
4. [4]
  Yuchen Zhou , Huanmin Liu , Hongxing Li , Xinyu Song , Yonghua Tang , Peng Zhou . Designing thermodynamically stable noble metal single-atom photocatalysts for highly efficient non-oxidative conversion of ethanol into high-purity hydrogen and value-added acetaldehyde. Acta Physico-Chimica Sinica, 2025, 41(6): 100067-0. doi: 10.1016/j.actphy.2025.100067
5. [5]
  Jie Li , Huida Qian , Deyang Pan , Wenjing Wang , Daliang Zhu , Zhongxue Fang . Efficient Synthesis of Anethaldehyde Induced by Visible Light. University Chemistry, 2024, 39(4): 343-350. doi: 10.3866/PKU.DXHX202310076
6. [6]
  Yongwei ZHANG , Chuang ZHU , Wenbin WU , Yongyong MA , Heng YANG . Efficient hydrogen evolution reaction activity induced by ZnSe@nitrogen doped porous carbon heterojunction. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 650-660. doi: 10.11862/CJIC.20240386
7. [7]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
8. [8]
  Liuyun Chen , Wenju Wang , Tairong Lu , Xuan Luo , Xinling Xie , Kelin Huang , Shanli Qin , Tongming Su , Zuzeng Qin , Hongbing Ji . Soft template-induced deep pore structure of Cu/Al₂O₃ for promoting plasma-catalyzed CO₂ hydrogenation to DME. Acta Physico-Chimica Sinica, 2025, 41(6): 100054-0. doi: 10.1016/j.actphy.2025.100054
9. [9]
  Ruyan Liu , Zhenrui Ni , Olim Ruzimuradov , Khayit Turayev , Tao Liu , Luo Yu , Panyong Kuang . Ni-induced modulation of Pt 5d-H 1s antibonding orbitals for enhanced hydrogen evolution and urea oxidation. Acta Physico-Chimica Sinica, 2025, 41(12): 100159-0. doi: 10.1016/j.actphy.2025.100159
10. [10]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
11. [11]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
12. [12]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
13. [13]
  Tianlong Zhang , Jiajun Zhou , Hongsheng Tang , Xiaohui Ning , Yan Li , Hua Li . Virtual Simulation Experiment for Laser-Induced Breakdown Spectroscopy (LIBS) Analysis. University Chemistry, 2024, 39(6): 295-302. doi: 10.3866/PKU.DXHX202312049
14. [14]
  Wenjian Zhang , Mengxin Fan , Wenwen Fei , Wei Bai . Cultivation of Critical Thinking Ability: Based on RAFT Polymerization-Induced Self-Assembly. University Chemistry, 2025, 40(4): 108-112. doi: 10.12461/PKU.DXHX202406099
15. [15]
  Jiahao Zeng , Hui Chao . 诱导程序性细胞死亡的金属抗肿瘤药物研究. University Chemistry, 2025, 40(6): 145-159. doi: 10.12461/PKU.DXHX202406019
16. [16]
  Junjian Wang , Qingquan Yu , Shunyao Liu , Yuke Chen , Xiaoyu Liu , Guodong Li , Xiaoyan Liu , Hong Liu , Weijia Zhou . Laser-Induced Carbonization of Hydroxyapatite Sandwich Paper for Inkless Printing. Acta Physico-Chimica Sinica, 2024, 40(4): 2304024-0. doi: 10.3866/PKU.WHXB202304024
17. [17]
  Pengli GUAN , Renhu BAI , Xiuling SUN , Bin LIU . Trianiline-derived aggregation-induced emission luminogen probe for lipase detection and cell imaging. Chinese Journal of Inorganic Chemistry, 2025, 41(9): 1817-1826. doi: 10.11862/CJIC.20250058
18. [18]
  Shengwen XU , Longlong YANG , Houji CAO , Deshuang TU , Xing WEI , Changsheng LU , Hong YAN . Research progress on light-induced functionalization of polyhedral carborane clusters. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2187-2200. doi: 10.11862/CJIC.20250192
19. [19]
  Ruoxi RUN , Jikai ZHU , Lixia HAN , Zhiyin XIAO , Xiujuan JIANG , Jing JIN . Red light-induced CO-release from manganese carbonyl complexes. Chinese Journal of Inorganic Chemistry, 2025, 41(12): 2575-2583. doi: 10.11862/CJIC.20250132
20. [20]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(690)
HTML views(85)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142