Citation: DONG Yu, YU Zhongming, LI Hongyu, ZHAO Lisha, SHOU Dan. Rat urinary metabolomics analysis of intervention of Phellinus igniarius based on ultra high performance liquid chromatography with high- definition mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(8): 811-816. doi: 10.3724/SP.J.1123.2016.04009 shu

Rat urinary metabolomics analysis of intervention of Phellinus igniarius based on ultra high performance liquid chromatography with high- definition mass spectrometry

DONG Yu ¹ ,
YU Zhongming ¹ ,
LI Hongyu ¹ ,
ZHAO Lisha ² ,
SHOU Dan ^1,,

1.
1. Key Laboratory of Research and Development of Traditional Chinese Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China;
2.
2. College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China

Corresponding author: SHOU Dan,
Received Date: 6 April 2016

Fund Project:

To investigate the influence of oral administration of decoction of Phellinus igniarius on the body, this study utilized ultra high performance liquid chromatography with high-definition mass spectrometry (UPLC-HDMS) technology to detect the change of metabolites in rat urine after oral decoction of Phellinus igniarius. Orthogonal partial least squares discriminant analysis (OPLS-DA) was adapted to analyze the metabolites of rat urine in the control group and dosed group, then the potential biomarkers were screened out. Finally the related metabolic pathways were analyzed by MetaboAnalyst 3.0 website. The results showed that urinary metabolites in the two groups were well separated at the 28th day, and 10 biomarkers were found and identified. After the oral administration of decoction of Phellinus igniarius, it mainly impacted the cysteine and methionine metabolism, arginine and proline metabolism, purine metabolism pathways. These results of the study would lay a foundation for further research on the pharmacodynamic mechanism of Phellinus igniarius.
- ultra high performance liquid chromatography (UPLC),
- high-definition mass spectrometry (HDMS),
- metabolomics,
- pattern recognition analysis,
- Phellinus igniarius,
- rat urine
1. [1]
  [1] Bao H Y, Wang C Y, Tu L G E. Mycosystema, 2013, 32(Suppl 1): 70 包海鹰, 王超儀, 图力古尔. 菌物学报, 2013, 32(Suppl 1): 70
2. [2]
  [2] Balakumar R, Sivaprakasam E, Kavitha D, et al. Int J Biosci, 2011, 1(3): 72
3. [3]
  [3] Kim H M, Lee D H. Korean J Mycol, 2010, 38(2): 179
4. [4]
  [4] Park B J, Lim Y S, Lee H J, et al. BMB Rep, 2009, 42(8): 500
5. [5]
  [5] Li S C, Yang X M, Ma H L, et al. Carbohyd Polym, 2015, 133: 24
6. [6]
  [6] Yuan C, Huang X, Cheng L, et al. Food Chem, 2009, 115(2): 581
7. [7]
  [7] Dong W, Ning L, Lu W, et al. World J Microb Biot, 2009, 25(4): 633
8. [8]
  [8] Zhao C, Liao Z, Wu X, et al. J Food Sci, 2014, 79(5): H1002
9. [9]
  [9] Noh J R, Lee I K, Ly S Y, et al. J Med Food, 2011, 14(3): 209
10. [10]
  [10] Wang J, Yuan Z M, Kong H W, et al. Chinese Journal of Chromatography, 2012, 30(1): 8 王静, 袁子民, 孔宏伟, 等. 色谱, 2012, 30(1): 8
11. [11]
  [11] Nicholson J K, Lindon J C, Holmes E. Xenobiotica, 1999, 29(11): 1181
12. [12]
  [12] Gao H, Xu Y Y, Sun L, et al. Chinese Journal of Chromatography, 2015, 33(6): 622 高慧, 许媛媛, 孙丽, 等. 色谱, 2015, 33(6): 622
13. [13]
  [13] Yang T Z, Luo P, Li Y L, et al. Chinese Journal of Chromatography, 2014, 32(2): 126 杨太忠, 罗萍, 李艳丽, 等. 色谱, 2014, 32(2): 126
14. [14]
  [14] Want E J, Perrine M, Filippos M, et al. Nat Protoc, 2012, 8(1): 17
15. [15]
  [15] Canuto G A B, Castilho-Martins E A, Tavares M F M, et al. Anal Bioanal Chem, 2014, 406(14): 3459
16. [16]
  [16] Liu J H, Sun H, Zhang A H, et al. Biomed Chromatogr, 2014, 28(4): 500
17. [17]
  [17] Fang Z Z, Gonzalez F J. Arch Toxicol, 2014, 88(8): 1491
18. [18]
  [18] Zhang A, Sun H, Dou S, et al. Analyst, 2013, 138(1): 353
19. [19]
  [19] Sun H, Wang M, Zhang A, et al. Phytochem Analysis, 2013, 24(3): 263
20. [20]
  [20] Tazuma S, Kanno K, Sugiyama A, et al. J Gastroen Hepatol, 2013, 28(Suppl 4): 103
21. [21]
  [21] Stincone A, Prigione A, Cramer T, et al. Biol Rev, 2015, 90(3): 927
22. [22]
  [22] Zhao C, Liao Z, Wu X, et al. J Food Sci, 2014, 79(5): 1002
23. [23]
  [23] Lu D, Cao J. J Shanghai Univ, 2001, 5(2): 164
24. [24]
  [24] Zhong Y, Huang Y, Huang Y, et al. Mutagenesis, 2010, 25(1): 83
1. [1]
  Qian Wu , Yuanxia Lv , Zixuan Guo , Zhihao Zhao , Zhimin Zhang , Hongmei Lu . A Case Study and Practice of Research-Oriented Comprehensive Instrumental Analysis Laboratory Courses. University Chemistry, 2025, 40(10): 194-202. doi: 10.12461/PKU.DXHX202411063
2. [2]
  Zhenjun Mao , Haorui Gu , Haiyan Che , Xufeng Lin . Exploration on Experiment Teaching of UHPLC-IC Based on Valve Switching Method. University Chemistry, 2024, 39(4): 81-86. doi: 10.3866/PKU.DXHX202311013
3. [3]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
4. [4]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
5. [5]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
6. [6]
  Haiyuan Wang , Xiaoning Jin , Yajing Sun , Zhen Zhang , Wentao Zhao , Yi Li . Practical Exploration of High-Performance Liquid Chromatography Experiment Teaching Reform Empowered by Artificial Intelligence. University Chemistry, 2026, 41(4): 45-51. doi: 10.12461/PKU.DXHX202505076
7. [7]
  Siwei Hou , Yaxin Niu , Guanglu Zhang , Yanmei Yang , Xu Wang , Zhenzhen Chen . Application of Solid-Phase Microextraction and Mass Spectrometry in Environmental Detection. University Chemistry, 2026, 41(3): 297-306. doi: 10.12461/PKU.DXHX202504078
8. [8]
  Linghua Chen . 基于双联动“三学”模式的食品专业分析化学教学改革. University Chemistry, 2025, 40(8): 78-91. doi: 10.12461/PKU.DXHX202409095
9. [9]
  Songmei Ma , Ying Zhang , Gang Liu , Wenlong Xu . Comprehensive Experiment Teaching Exploration and Practice in Polymeric Materials Integrating Research-Driven Learning, Creativity-Enhanced Competency, and Science-Education Synergy: A Case Study of Machine Learning-Assisted Intelligent Handwriting Recognition System. University Chemistry, 2026, 41(1): 289-297. doi: 10.12461/PKU.DXHX202509083
10. [10]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
11. [11]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
12. [12]
  Yujing Chen , Hongqun Ouyang , Dan Zhao , Yanyan Chu , Zhengping Qiao . Recommendations for the Content and Instruction of the Physical Chemistry Experiment “Construction of Ternary Liquid-Liquid Phase Diagrams”. University Chemistry, 2025, 40(7): 359-366. doi: 10.12461/PKU.DXHX202409120
13. [13]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
14. [14]
  Yan Long , Wenbo Zhao , Qing Cao , Xiangyu Li , Fukui Li , Yanwei Hu , Shiyu Song , Kaikai Liu . Phosphorescent carbon nanodot inks for scalable and high-resolution invisible printing. Acta Physico-Chimica Sinica, 2026, 42(3): 100198-0. doi: 10.1016/j.actphy.2025.100198
15. [15]
  Yue-Zhou Zhu , Kun Wang , Shi-Sheng Zheng , Hong-Jia Wang , Jin-Chao Dong , Jian-Feng Li . Application and Development of Electrochemical Spectroscopy Methods. Acta Physico-Chimica Sinica, 2024, 40(3): 2304040-0. doi: 10.3866/PKU.WHXB202304040
16. [16]
  Houjin Li , Lin Wu , Xingwen Sun , Yuan Zheng , Zhanxiang Liu , Shuanglian Cai , Ying Xiong , Guangao Yu , Qingwen Liu , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Jianrong Zhang , Shuyong Zhang . Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments. University Chemistry, 2025, 40(5): 93-105. doi: 10.12461/PKU.DXHX202408100
17. [17]
  Daxin Liang , Yudong Li , Haiyue Yang , Bailing Chen , Zhiming Liu , Chengyu Wang . Reform and Practice of an AI-Empowered “Learning-Understanding-Application” Training Model in Materials Chemistry Education. University Chemistry, 2025, 40(9): 253-263. doi: 10.12461/PKU.DXHX202503020
18. [18]
  Xiaoqi LAN , Wei LI , Long JING , Mengyu SU , Xiaoling LUO , Zheng LIU , Qun TANG . Synthesis, crystal structure, and spectral properties of transition-metal-organic frameworks based on thiophene carboxylic acid ligands. Chinese Journal of Inorganic Chemistry, 2026, 42(2): 309-316. doi: 10.11862/CJIC.20250212
19. [19]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
20. [20]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1098)
HTML views(154)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142