Citation: SONG Qingqing, LIU Yao, ZHANG Lingling, ZHOU Li, TU Pengfei, SONG Yuelin. Simultaneous determination of three phenylethanoid glycosides in Cistanche tubulosa by online pressurized liquid microextraction-turbulent flow chromatography-high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2016, 34(6): 572-576. doi: 10.3724/SP.J.1123.2016.02014 shu

Simultaneous determination of three phenylethanoid glycosides in Cistanche tubulosa by online pressurized liquid microextraction-turbulent flow chromatography-high performance liquid chromatography

SONG Qingqing ^1,2 ,
LIU Yao ^1,2 ,
ZHANG Lingling ^1,2 ,
ZHOU Li ^1,2 ,
TU Pengfei ¹ ,
SONG Yuelin ^1,*,,

1.
Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
2.
School of Chinese Meteria Medica, Beijing University of Chinese Medicine, Beijing 100102, China

Corresponding author: SONG Yuelin, yhdql@tust.edu.cn
Received Date: 20 February 2016

Fund Project: Quality Guarantee System of Chinese Herbal Medicines 201507002National Natural Science Fundation of China 81403073

An online pressurized liquid microextraction-turbulent flow chromatography-high performance liquid chromatography (online PLME-TFC-HPLC) platform was configured for simultaneous determination of three phenylethanoid glycosides in Cistanche tubulosa. Micro amount powder of crude material (0.5 mg) was mixed with clean diatomaceous earth and packed into a vessel which then was put into a hollow guard column. To generate high temperature and high pressure, a long polyetheretherketone (PEEK) tube (1000 mm×0.13 mm) was linked to the end of the hollow guard column that was warmed in the column oven (70℃). The water containing 0.1% (v/v) formic acid acted as the extraction solvent and was delivered at 2.5 mL/min. Two electronic 6-port/2-channel valves were responsible for dividing the whole program into extraction and elution phases. The analytes were purified and enriched in a TurboFlow cyclone column, and back-flushed onto a Capcell PAK C₁₈ AQ column under a gradient elution program with 0.1% (v/v) aqueous formic acid-acetonitrile at the elution phase. The ultraviolet wavelength was set at 340 nm to monitor phenylethanoid glycosides. The calibration curves for the three phenylethanoid glycosides revealed good linearities in the range of 1-200 mg/L (r>0.999). The limits of quantification (LOQs) were 0.50 mg/L (echinacoside), 0.25 mg/L (acteoside) and 0.38 mg/L (isoacteoside). The spiked recoveries were in the range of 83.13%-114.00% with the relative standard deviations (RSDs) between 1.89% and 13.34%. All the results indicated that this method is facile, efficient, reliable, and advantageous at time, labor, solvent, and material savings. The method also reduces the degradation risks and is suitable for the determination of phenylethanoid glycosides in Cistanche tubulosa.
1. [1]
  Tu P F, Chen Q L, Jiang Y, et al.. Modern Chinese Medicine, 2015, 17(4):349
2. [2]
  Mustafa A, Turner C.. Anal Chim Acta, 2011, 703(1):8
3. [3]
  Ramos L, Kristenson E M, Brinkman U A.. J Chromatogr A, 2002, 975:3
4. [4]
  Camel V.. Analyst, 2001, 126(3):1182
5. [5]
  Liu Y, Xiu X Y, Wang W Y.. Journal of Anhui Agricultural University, 2015, 42(2):294
6. [6]
  Lu D Y, Zhang J Y, Yang Z Y, et al.. J Sep Sci, 2013, 36(12):1945
7. [7]
  Guo W H.. World Journal of Intergrated Traditional and Western Medicine, 2014, 9(5):496
8. [8]
  Tian Y B, Yang G A, Huang W J, et al.. Journal of Anhui Agricultural Sciences, 2015, 43(33):171
9. [9]
  Zhou Y, Li W, Han L F, et al.. Spectroscopy and Spectral Analysis, 2015, 35(4):1056
10. [10]
  Zhang L, Kong X H, He Q, et al.. Chinese Journal of Chromatography, 2014, 32(6):559
11. [11]
  Bousova K, Senyuva H, Mittendorf K.. J Chromatogr A, 2013, 1274:19
12. [12]
  Guo F, Shao J, Liu Q, et al.. Talanta, 2014, 125(11):432
13. [13]
  Marzinke M A, Breaud A R, Clarke W.. Clin Chim Acta, 2013, 417:12
14. [14]
  Carr A G, Mammucari R, Foster N R.. Chem Eng J, 2011, 172(1):1
15. [15]
  Kilpelainen P O, Hautala S S, Byman O O, et al.. Green Chem, 2014, 16(6):3186
16. [16]
  Pharmacopoeia Commission of People's Republic of China. Pharmacopoeia of People's Republic of China, Part 1[J]. Beijing:Chinese Medical Science and Technology Press, 2010126.
1. [1]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
2. [2]
  Haiyuan Wang , Xiaoning Jin , Yajing Sun , Zhen Zhang , Wentao Zhao , Yi Li . Practical Exploration of High-Performance Liquid Chromatography Experiment Teaching Reform Empowered by Artificial Intelligence. University Chemistry, 2026, 41(4): 45-51. doi: 10.12461/PKU.DXHX202505076
3. [3]
  Tong Wang , Liangyu Hu , Shiqi Chen , Xinqiang Fu , Rui Wang , Kun Li , Shuangyan Huan . Determination of Benzenediol Isomers in Cosmetics Using High-Performance Liquid Chromatography Empowered by “Mathematical Separation”. University Chemistry, 2026, 41(1): 9-19. doi: 10.12461/PKU.DXHX202503128
4. [4]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
5. [5]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
6. [6]
  Gengjia Chen , Junjie Ou . Application of the van Deemter Equation in Instrumental Analysis Teaching: A Case of Organic Polymer Monolithic Columns. University Chemistry, 2025, 40(11): 362-368. doi: 10.12461/PKU.DXHX202502003
7. [7]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
8. [8]
  Lingyu Chang , Yanfang Lang , Yuyan Zhu , Jie Wang , Ying Guo , Die Wang , Peng Ding , Yueming Zhou , Zhixiang Gong , Shujuan Liu . Machine Learning-Optimized Microcolumn Ion Exchange Chromatography for Trace Arsenic Determination. University Chemistry, 2026, 41(1): 76-84. doi: 10.12461/PKU.DXHX202506023
9. [9]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
10. [10]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
11. [11]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
12. [12]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174
13. [13]
  Houjin Li , Lin Wu , Xingwen Sun , Yuan Zheng , Zhanxiang Liu , Shuanglian Cai , Ying Xiong , Guangao Yu , Qingwen Liu , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Jianrong Zhang , Shuyong Zhang . Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments. University Chemistry, 2025, 40(5): 93-105. doi: 10.12461/PKU.DXHX202408100
14. [14]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
15. [15]
  Chunhui Gao , Lurong Li , Guanwei Peng , Jinni Shen , Wenxin Dai , Zizhong Zhang . Efficient photocatalytic NADH regeneration and enzymatic CO₂ reduction over[Cp^*Rh(bpy)H₂O]²⁺ self-assembled CdIn₂S₄ flower-like microspheres. Acta Physico-Chimica Sinica, 2026, 42(3): 100165-0. doi: 10.1016/j.actphy.2025.100165
16. [16]
  Zeyi Yan , Ruitao Liu , Xinyu Qi , Yuxiang Zhang , Lulu Sun , Xiangyuan Li , Anchao Feng . Exploration of Suspension Polymerization: Preparation and Fluorescence Stability of Perovskite Polystyrene Microbeads. University Chemistry, 2025, 40(4): 72-79. doi: 10.12461/PKU.DXHX202405110
17. [17]
  Lijun Yue , Siya Liu , Peng Liu . 不同晶相纳米MnO₂的制备及其对生物乙醇选择性氧化催化性能的测试——一个科研转化的综合化学实验. University Chemistry, 2025, 40(8): 225-232. doi: 10.12461/PKU.DXHX202410005
18. [18]
  Chang Guo , Haipeng Yang , Hui Fang , Yingguo Zhao , Yating Li . 基于深度学习的物理化学课程DOK教学实践初探——以弯曲液面附加压力和蒸气压教学为例. University Chemistry, 2025, 40(6): 28-36. doi: 10.12461/PKU.DXHX202408049
19. [19]
  Jiandong Liu , Xin Li , Daxiong Wu , Huaping Wang , Junda Huang , Jianmin Ma . Anion-Acceptor Electrolyte Additive Strategy for Optimizing Electrolyte Solvation Characteristics and Electrode Electrolyte Interphases for Li||NCM811 Battery. Acta Physico-Chimica Sinica, 2024, 40(6): 2306039-0. doi: 10.3866/PKU.WHXB202306039
20. [20]
  Xiaojun Liu , Lang Qin , Yanlei Yu . Dynamic Manipulation of Photonic Bandgaps in Cholesteric Liquid Crystal Microdroplets for Applications. Acta Physico-Chimica Sinica, 2024, 40(5): 2305018-0. doi: 10.3866/PKU.WHXB202305018

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(941)
HTML views(115)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142