Citation: CHEN Zhaojie, HUANG Huili, WEI Jie, LI Xuesheng. Application of chromatography-mass spectrometry technology in the component identification and fingerprint establishment of the three kinds of pomelo in China[J]. Chinese Journal of Chromatography, ;2016, 34(6): 558-566. doi: 10.3724/SP.J.1123.2016.01033 shu

Application of chromatography-mass spectrometry technology in the component identification and fingerprint establishment of the three kinds of pomelo in China

1.
Institute of Pesticide & Environmental Toxicology, Guangxi University, Nanning 530005, China

Corresponding author: LI Xuesheng, lxsnngx@163.com
Received Date: 28 January 2016

Fund Project: Guangxi Special Invited Scientist Program No.2013B015Natural Science Foundation of China No.31101297Scientific Fund of Guangxi University No.XBZ110383

The chemical constituents in different tissues (primary cuticula, epidermis, peel, pulp, seed) of the three kinds of pomelo (Shatian pomelo, Guanxi honey pomelo, Yuhuan pomelo) in China were qualitatively and quantitatively analyzed by gas chromatography-mass spectrometry, liquid chromatography-electrospray ion trap-mass spectrometry and ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. The results showed that 68 different chemical constituents were identified in the five tissues of the three kinds of pomelo (19 alcohols, 15 esters, 9 olefins, 8 organic acids, 7 ketones, 4 alkanes and 6 other substances). Among which 7 common constituents were found in primary cuticula, and the highest content was 44.03%(limonene). In epidermis, the highest content in 6 common constituents was 44.62%(oleic acid amide). In 10 common constituents of peel, the highest content was 30.75%(sitosterol). The highest content was 20.02% (palmitic acid) of 11 common constituents in pulp. In seed, the highest content of 15 common constituents was 45.83%( α-linoleic acid). We also applied the cluster analysis and similarity analysis to the chemical constituents of different parts of the three kinds of pomelo. It is apparent that there are differences and similarities in species and contents of the constituents in the same parts of different varieties of pomelo. The chromatographic fingerprint specificity is obvious, which is helpful to put forward a new idea of chromatographic fingerprint in classification, quality control and geographical tracing of pomelo.
- chromatography-mass spectrometry,
- chemical constituents,
- pomelo,
- analysis of the divergences,
- fingerprint,
- cluster analysis
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