Citation: LIN Qi, LI Guobo, GE Pin, XU Rongxian, LIN Guobin. Determination of trans-fatty acid isomers in human milk fat by gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(5): 520-527. doi: 10.3724/SP.J.1123.2016.01003 shu

Determination of trans-fatty acid isomers in human milk fat by gas chromatography-mass spectrometry

LIN Qi ¹ ,
LI Guobo ² ,
GE Pin ² ,
XU Rongxian ³ ,
LIN Guobin ^1,,

1.
1. Fujian Provincial Key Laboratory of Zoonosis Research(Fujian Center for Disease Control & Prevention), Fuzhou 350001, China;
2.
2. Fujian Maternal and Child Health Care Hospital(Fujian Women and Children's Hospital), Fuzhou 350001, China;
3.
3. Public Health School of Fujian Medical University, Fuzhou 350108, China

Corresponding author: LIN Guobin,
Received Date: 3 January 2016

Fund Project: 福建省科技重大专项(2013YZ0002-1) (2013YZ0002-1)福建省医药创新课题项目(2014-CXB-7). (2014-CXB-7)

A method for the determination of trans-fatty acid isomers in human milk fat was developed by gas chromatography-mass spectrometry (GC-MS), and applied to analyze TFAs in human milk fat. The fat was extracted with diethylether and petroleum ether after ammonia hydrolysis of human milk. C21: 0 internal standard and boron trifluoride methanol solution were added to the extract for fat esterification. The solution then was refluxed in 80℃ water bath for 15 min. Finally the trans-fatty acid methyl ester isomers were extracted with hexane, and analyzed by GC-MS. GC conditions were as follows: an HP-88 column (100 m×0.25 mm×0.2 μ m) with inlet temperature of 260℃, at a split ratio of 10: 1, and a He flow rate of 1 mL/min. The initial column temperature was 140℃ (held for 5 min), then raised up to 240℃ at a rate of 4℃/min (held for 15 min). The electronic ionization (EI) source energy was 70 eV, with auxiliary (AUX) temperature of 280℃, ion source temperature of 230℃, quadrupole temperature of 150℃ in selected ion monitoring (SIM) mode. This method can analyze the 18 TFAs. The method detection limits (MDLs) of 12 TFAs were 4.0-47.1 mg/kg, and the average recoveries were 80%-113% with the relative standard deviation (RSD, n=6) range of 2.9%-14.5%. TFAs were detected in some real samples and the contents were 9.5-46.9 mg/kg. The method is reliable and sensitive, and would be better if the background interference of fatty acids should be diminished by silver ion solid phase extraction.
- gas chromatography-mass spectrometry (GC-MS),
- trans-fatty acids (TFAs),
- human milk,
- fat
1. [1]
  [1] Liu Y, Liu H C, Shi J X. Journal of Food Safety and Quality, 2015, 6(8): 3160 刘莹, 刘会昌, 石建新. 食品安全质量检测学报, 2015, 6(8): 3160
2. [2]
  [2] Lin Q, Xu R X. Strait Journal of Preventive Medicine, 2012, 18(3): 23 林麒, 许榕仙. 海峡预防医学杂志, 2012, 18(3): 23
3. [3]
  [3] Szabo E, Boehm G, Beermann C, et al. Am J Clin Nutr, 2007, 85(5): 1320
4. [4]
  [4] Cohen J F, Rifas-Shiman S L, Rimm E B, et al. Am J Clin Nutr, 2012, 94(5): 1241
5. [5]
  [5] Morrison J A, Glueck C J, Wang P. Fertil Steril, 2008, 90(2): 385
6. [6]
  [6] Pimentel G D, Lira F S, Rosa J C, et al. J Nutr Biochem, 2011, 23(3): 265
7. [7]
  [7] Zhao G Z, Liu X L, Liu Z F. Cereals & Oils, 2007(1): 7 赵国志, 刘喜亮, 刘智锋. 粮食与油脂, 2007(1): 7
8. [8]
  [8] Anderson A K, Mcdougald D M, Steiner-Asiedu M. Eur J Clin Nutr, 2010, 64(11): 1308
9. [9]
  [9] Wijga A H, van Houwelingen A C, Kerkhof M, et al. J Allergy Clin Immunol, 2006, 117(2): 440
10. [10]
  [10] Salam M T, Li Y F, Langholz B, et al. J Asthma, 2005, 42(6): 513
11. [11]
  [11] Chen X, Shi A M, Liu H Z, et al. Food Science, 2015, 36(21): 291 陈雪, 石爱民, 刘红芝, 等. 食品科学, 2015, 36(21): 291
12. [12]
  [12] Wu Y W, Li B N, Wang Y, et al. Journal of Food Safety & Quality, 2015, 6(9): 3329 武彦文, 李冰宁, 汪雨, 等. 食品安全质量检测学报, 2015, 6(9): 3329
13. [13]
  [13] Xie M Y, Yang M Y, Nie S P. Chinese Journal of Chromatography, 2011, 29(7): 601 谢明勇, 杨美艳, 聂少平. 色谱, 2011, 29(7): 601
14. [14]
  [14] Tyburczy C, Mossoba M M, Rader J I. Anal Bioanal Chem, 2013, 405(17): 5759
15. [15]
  [15] Li R, Xu X M, Li Y L, et al. Chinese Journal of Chromatography, 2010, 28(12): 1168 李蕊, 徐小民, 李亚利, 等. 色谱, 2010, 28(12): 1168
16. [16]
  [16] Li J, Fan Y, Zhang Z, et al. Lipids, 2009, 44(3): 257
17. [17]
  [17] Daud A Z, Mohd-Esa N, Azlan A, et al. Asia Pac J Clin Nutr, 2013, 22(3): 431
18. [18]
  [18] Wu H Q, Huang X L, Lin X S, et al. Chinese Journal of Analytical Chemistry, 2007, 35(7): 998 吴惠勤, 黄晓兰, 林晓珊, 等. 分析化学, 2007, 35(7): 998
19. [19]
  [19] GB 5413.3-2010
20. [20]
  [20] Li P W, Xie L H, Wei L F, et al. Chinese Journal of Oil Crop Sciences, 2009, 31(3): 374 李培武, 谢立华, 魏丽芳, 等. 中国油料作物学报, 2009, 31(3): 374
21. [21]
  [21] Han L, Huang J, Ni X L, et al. Chinese Journal of Health Laboratory Technology, 2007, 17(7): 1155 韩丽, 黄杰, 倪昕路, 等. 中国卫生检验杂志, 2007, 17(7): 1155
22. [22]
  [22] Wang Y J, Na G S, Wang Z, et al. Chemical Analysis and Meterage, 2012, 21(5): 85 王艳洁, 那广水, 王震, 等. 化学分析计量, 2012, 21(5): 85
23. [23]
  [23] Environmental Protection Agency. Open-File Report 99-193. [2016-02-26]. http://water.usgs.gov/owq/OFR_99-193/
24. [24]
  [24] Lin Q, Li G B, Ge P, et al. Chinese Preventive Medicine, 2014, 15(7): 663 林麒, 李国波, 葛品, 等. 中国预防医学杂志, 2014, 15(7): 663
1. [1]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
2. [2]
  Pengli GUAN , Renhu BAI , Xiuling SUN , Bin LIU . Trianiline-derived aggregation-induced emission luminogen probe for lipase detection and cell imaging. Chinese Journal of Inorganic Chemistry, 2025, 41(9): 1817-1826. doi: 10.11862/CJIC.20250058
3. [3]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
4. [4]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
5. [5]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
6. [6]
  Yukun Chang , Haoqin Huang , Baolei Wang . Preparation of Trans-Cinnamic Acid via “One-Pot” Protocol of Aldol Condensation-Hydrolysis Reaction: Recommending an Improved Organic Synthesis Experiment. University Chemistry, 2024, 39(4): 322-328. doi: 10.3866/PKU.DXHX202309095
7. [7]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
8. [8]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
9. [9]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036
10. [10]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
11. [11]
  Yuanchun Pan , Xinyun Lin , Leyi Yang , Wenya Hu , Dekui Song , Nan Liu . Artificial Intelligence Science Practice: Preparation of Electronic Skin by Chemical Vapor Deposition of Graphene. University Chemistry, 2025, 40(11): 272-280. doi: 10.12461/PKU.DXHX202412052
12. [12]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
13. [13]
  Yu Peng , Jiawei Chen , Yue Yin , Yongjie Cao , Mochou Liao , Congxiao Wang , Xiaoli Dong , Yongyao Xia . Tailored cathode electrolyte interphase via ethylene carbonate-free electrolytes enabling stable and wide-temperature operation of high-voltage LiCoO₂. Acta Physico-Chimica Sinica, 2025, 41(8): 100087-0. doi: 10.1016/j.actphy.2025.100087
14. [14]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
15. [15]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
16. [16]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
17. [17]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174
18. [18]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
19. [19]
  Qingying Gao , Tao Luo , Jianyuan Su , Chaofan Yu , Jiazhu Li , Bingfei Yan , Wenzuo Li , Zhen Zhang , Yi Liu . Refinement and Expansion of the Classic Cinnamic Acid Synthesis Experiment. University Chemistry, 2024, 39(5): 243-250. doi: 10.3866/PKU.DXHX202311074
20. [20]
  Lanjun Cheng , Xinyuan Wang , Jie An , Xiang Wu , Chengfeng Zhu , Yanming Fu , Yougui Li . Improvement of the Resolution Experiment of Racemic Tartaric Acid. University Chemistry, 2025, 40(7): 277-285. doi: 10.12461/PKU.DXHX202408010

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(375)
HTML views(11)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142