Citation: ZHENG Yang, XU Xiuli, JI Shunli, YUAN Fei, HUANG Zhiqiang, YANG Bingcheng, ZHANG Feng. Analysis of 23 esters of flavor additive in tobacco products by gas chromatography-tandem mass spectrometry coupled with solid-phase extraction[J]. Chinese Journal of Chromatography, ;2016, 34(5): 512-519. doi: 10.3724/SP.J.1123.2015.12020 shu

Analysis of 23 esters of flavor additive in tobacco products by gas chromatography-tandem mass spectrometry coupled with solid-phase extraction

ZHENG Yang ^1,2 ,
XU Xiuli ¹ ,
JI Shunli ² ,
YUAN Fei ¹ ,
HUANG Zhiqiang ³ ,
YANG Bingcheng ^2,, ,
ZHANG Feng ^1,,

1.
1. Institute of Tobacco Safety and Control/Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100176, China;
2.
2. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
3.
3. Hunan Academy of Science and Technology for Inspection and Quarantine, Changsha 410004, China

Corresponding author: YANG Bingcheng, ZHANG Feng,
Received Date: 17 December 2015

Fund Project: 质检公益性行业科研专项(201410088, 201310134) (201410088, 201310134)中国检验检疫科学研究院基本科研业务费项目(2016JK009, 2015JK008) (2016JK009, 2015JK008)国家质检总局科研计划项目(2014IK084) (2014IK084)国家重大仪器设备开发专项(2012YQ14000806). (2012YQ14000806)

An analytical method for the simultaneous determination of 23 esters of flavor additive in tobacco products was established based on optimized solid-phase extraction (SPE) with gas chromatography-tandem mass spectrometry (GC-MS/MS). The samples were extracted with ethyl acetate, cleaned up by an amino SPE column. After conditioning step, the extracts were eluted by 4 mL ethyl acetate, and then were determined by GC-MS/MS in multi-reaction monitoring (MRM) mode. The results showed that all the 23 ester flavor additives had good linearity in the range of 20.0-500.0 μ g/L, with the correlation coefficients (r²) better than 0.996. The limits of detection (LODs) and limits of quantitation (LOQs) of these additives were in the ranges of 1.9-13.6 μ g/kg and 6.3-45.3 μ g/kg, respectively. The average recoveries of the 23 analysts were in the range of 62.1%-91.4% at the spiked levels of 1, 1.5, 2 times LOQ with relative standard deviations (RSDs) typically lower than 7%. The accurate, reliable and sensitive method can be applied to the determination of the 23 esters of flavor additive in tobacco products for rapid screening and quantitative analysis. The method also offered an opportunity to detect more ester additives that may be added in tobacco products.
- solid phase extraction (SPE),
- gas chromatography-tandem mass spectrometry (GC-MS/MS),
- ester flavor additives,
- tobacco products
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