Citation: WANG Li, SHAO Yingying, LI Lei, WANG Zhixu. Determination of vitamin A reserved in human liver by stable isotope dilution technique[J]. Chinese Journal of Chromatography, ;2016, 34(5): 533-537. doi: 10.3724/SP.J.1123.2015.12019 shu

Determination of vitamin A reserved in human liver by stable isotope dilution technique

WANG Li ¹ ,
SHAO Yingying ² ,
LI Lei ¹ ,
WANG Zhixu ^2,,

1.
1. Department of Hygienic Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing 211166, China;
2.
2. Department of Maternal, Child and Adolescent Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China

Corresponding author: WANG Zhixu,
Received Date: 17 December 2015

Fund Project: 国家自然科学基金项目(3097248,81172654) (3097248,81172654)江苏省高校优势学科建设工程项目(PAPD2010). (PAPD2010)

An analytical method for the determination of vitamin A (VA) reserved in human liver by stable isotope dilution technique and high performance liquid chromatography-gas chromatography/negative ion chemical ionization/mass spectrometry (HPLC-GC/NCI/MS) was developed. Before the test, deuterium labelled retinol acetate (²H₈-RAC) (1 mg) was ingested by the volunteers. After 21 days, the blood samples were collected and the serum was separated. The VA was extracted by n-hexane and purified by HPLC. Then the purified VA was dried under nitrogen for further derivatization. The derivative was finally detected by GC/NCI/MS. The concentration of the VA in the liver was calculated by using the formula of Furr-Olson. Under the optimized conditions, more than 85% of the VA in the serum samples could be collected, purified and detected. The detection precision (relative standard deviation, RSD, n=6) was less than 10% and the quantitative limit reached 26.4 μ g/L, which met the marked and unmarked VA detection requirements. Compared with the current domestic VA evaluation method, this method can more objectively reflect human VA nutrition level. When this determination technology combines with VA intervention experiment, it could evaluate a dietary intake level of VA to maintain a stability reserve level. At the same time, it also plays an important role in the research on biological conversion efficiency of carotene ingestion.
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