Citation: WU Haiyan, GUO Mengmeng, ZHAO Chunxia, TAN Zhijun, GU Haifeng, ZHAI Yuxiu, LU Lina. Liquid chromatography-tandem mass spectrometry analysis for azaspiracids and their metabolites[J]. Chinese Journal of Chromatography, ;2016, 34(4): 401-406. doi: 10.3724/SP.J.1123.2015.11043 shu

Liquid chromatography-tandem mass spectrometry analysis for azaspiracids and their metabolites

WU Haiyan ^1,2 ,
GUO Mengmeng ^1,2 ,
ZHAO Chunxia ^1,2 ,
TAN Zhijun ^1,2,, ,
GU Haifeng ³ ,
ZHAI Yuxiu ^1,2 ,
LU Lina ^1,2

1.
1. Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2.
2. Laboratory of Quality & Safety Risk Assessment for Aquatic Products(Qingdao), Ministry of Agriculture, Qingdao 266071, China;
3.
3. Third Institute of Oceanography, State Oceanic Administration, Xiamen 361000, China

Corresponding author: TAN Zhijun,
Received Date: 26 November 2015

Fund Project: 国家自然科学基金项目(41106109) (41106109)中央级基本科研业务费项目(20603022011004,20603022015017-2). (20603022011004,20603022015017-2)

A liquid chromatography-tandem mass spectrometry method was developed for the analysis of azaspiracids and their metabolites. Both target metabolites and non-objective compounds detection methods were investigated, and the secondary mass spectra were compared with the standard spectra libraries to achieve high precision qualitative analysis. As a result, 11 metabolites including AZA-2, 3, 6, 11, 12, 16, 17, 28 and 36, as well as the glutathione conjugated metabolites of AZAs were analyzed in toxin-producing algae, accumulated samples of metabolic experiments and positive samples. Conventional metabolites were unbiased detected by target metabolites detection method, and showed a better response to low concentration metabolites. The method has excellent reproducibility, and is easy for operators. It can be used for the routine monitoring of AZAs.
- liquid chromatography-tandem mass spectrometry (LC-MS/MS),
- azaspiracids (AZAs),
- metabolites,
- qualitative analysis
1. [1]
  [1] Yao J H, Tan Z J, Zhou D Q, et al. Chinese Journal of Chromatography, 2010, 28(4):363 姚建华, 谭志军, 周德庆, 等. 色谱, 2010, 28(4):363
2. [2]
  [2] Wu H, Guo M, Tan Z, et al. J Chromatogr A, 2014, 1358:172
3. [3]
  [3] Krock B, Tillmann U, Witt M, et al. Harmful Algae, 2014, 36:22
4. [4]
  [4] Gu H, Luo Z, Krock B, et al. Harmful Algae, 2013, 21/22:64
5. [5]
  [5] Furey A, O'Doherty S, O'Callaghan K, et al. Toxicon, 2010, 56(2):173
6. [6]
  [6] EFSA. The EFSA Journal, 2008, 723:1
7. [7]
  [7] Salas R, Tillmann U, John U, et al. Harmful Algae, 2011, 10(6):774
8. [8]
  [8] Jauffrais T, Marcaillou C, Herrenknecht C, et al. Toxicon, 2012, 60(4):582
9. [9]
  [9] Jauffrais T, Contreras A, Herrenknecht C, et al. Aquatic Toxicol, 2012, 124/125:179
10. [10]
  [10] Jauffrais T, Kilcoyne J, Herrenknecht C, et al. Toxicon, 2013, 65:81
11. [11]
  [11] EFSA. The EFSA Journal, 2009, 1306:1
12. [12]
  [12] Fang L Y, Yao X P, Wang L, et al. Chinese Journal of Health Laboratory Technology, 2014, 24(16):2316 方兰云, 姚浔平, 王立, 等. 中国卫生检验杂志, 2014, 24(16):2316
13. [13]
  [13] Han S, Liu X, Li J H, et al. Food Science, 2014, 35(4):116 韩深, 刘鑫, 李建辉, 等. 食品科学, 2014, 35(04):116
14. [14]
  [14] Han S, Wang P Y, Liu Y, et al. Chinese Journal of Chromatography, 2013, 31(10):939 韩深, 王珮玥, 刘萤, 等. 色谱, 2013, 31(10):939
15. [15]
  [15] Botana L M. Seafood and Freshwater Toxins:Pharmacology, Physiology, and Detection. 3rd ed. Florida:CRC Press, 2014
16. [16]
  [16] Rehmann N, Hess P, Quilliam M A. Rapid Commun Mass Spectrom, 2008, 22(4):549
17. [17]
  [17] Chen T, Xu X, Wei J, et al. PLoS One, 2013, 8(5):1
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