Citation: XU Xu, XIAO Yuancan, GENG Dandan, PI Li, DONG Qi, HU Fengzu. Simultaneous determination of 16 sulfonamide residues in beef by high performance liquid chromatography-fluorescence detection with online post-column derivatization[J]. Chinese Journal of Chromatography, ;2016, 34(4): 422-428. doi: 10.3724/SP.J.1123.2015.11020 shu

Simultaneous determination of 16 sulfonamide residues in beef by high performance liquid chromatography-fluorescence detection with online post-column derivatization

XU Xu ^1,2 ,
XIAO Yuancan ^1,, ,
GENG Dandan ^1,2 ,
PI Li ¹ ,
DONG Qi ¹ ,
HU Fengzu ¹

1.
1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China;
2.
2. University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: XIAO Yuancan,
Received Date: 12 November 2015

Fund Project: 中国科学院仪器功能开发技术创新项目(Ig201308) (Ig201308)中国科学院知识创新工程重要方向项目(KSCX2-EW-J-26) (KSCX2-EW-J-26)青海省科技计划项目(2015-ZL-Y23) (2015-ZL-Y23)青海省科技平台建设项目(2012-T-Y19). (2012-T-Y19)

In this study, we investigated the effects of the four kinds of derivatization reagents, including fluorescamine, o-phthaladehyde, fluorescein isothiocyanate isomer and 2, 3-naphthalenedicarboxaldehyde. A high performance liquid chromatography with fluorescence detection method for the rapid determination of 16 sulfonamide residues in beef was developed by using improved QuEChERS method for sample pretreatment and fluorescamine for online post-column derivatization. The beef samples were extracted with acetonitrile containing 1%(v/v) acetic acid, cleaned-up by QuEChERS method, injected for online post-column derivatization with fluorescamine, and then analysed with fluorescence detection. Under the optimized conditions of QuEChERS and chromatography, the 16 sulfonamide residues showed good linearities in the range of 0.024-2.533 mg/L with the correlation coefficients (r) higher than 0.992. The limits of detection of the method were 1.6 to 8.2 μg/kg. The average recoveries of the 16 sulfonamide residues were in the range of 66.6%-109.5% with the relative standard deviations from 0.9% to 9.9%. The method has the advantages of rapidity, simplicity, high sensitivity and better purification effect. It is suitable for the rapid determination of the sulfonamide residues in beef.
- QuEChERS,
- fluorescamine,
- online post-column derivatization,
- high performance liquid chromatography-fluorescence detection (HPLC-FLR),
- sulfonamides,
- beef
1. [1]
  [1] Baran W, Adamek E, Ziemiańska J, et al. J Hazard Mater, 2011, 196:1
2. [2]
  [2] Zhao H X, Liu H P, Yan Z Y. Chinese Journal of Chromatography, 2014, 32(3):294 赵海香, 刘海萍, 闫早婴. 色谱, 2014, 32(3):294
3. [3]
  [3] Abdallah H, Arnaudguilhem C, Jaber F, et al. J Chromatogr A, 2014, 1355:61
4. [4]
  [4] Cheng G D, Wu X H, Jin Z, et al. Chinese Journal of Chromatography, 2015, 33(8):892 程国栋, 吴小慧, 金珠, 等. 色谱, 2015, 33(8):892
5. [5]
  [5] Nebot C, Regal P, Miranda J, et al. J Chromatogr Sci, 2012, 50:414
6. [6]
  [6] Huang D M, Huang X Y, Gu R R, et al. Chinese Journal of Chromatography, 2014, 32(8):874 黄冬梅, 黄宣运, 顾润润, 等. 色谱, 2014, 32(8):874
7. [7]
  [7] Mor F, Sahindokuyucu Kocasari F, Ozdemir G, et al. Food Chem, 2012, 134:1645
8. [8]
  [8] Gao Y Y, Zhang C H, Liu X, et al. Chinese Journal of Chromatography, 2014, 32(5):524 高洋洋, 张朝晖, 刘鑫, 等. 色谱, 2014, 32(5):524
9. [9]
  [9] Reig M, Toldrá F. Meat Sci, 2008, 78:60
10. [10]
  [10] Bernal J, Nozal M J, Jiménez J J, et al. J Chromatogr A, 2009, 1216:7275
11. [11]
  [11] Cai Z X, Zhang Y, Pan H F, et al. J Chromatogr A, 2008, 1200:144
12. [12]
  [12] Zou Q H, Xie M X, Wang X F, et al. J Sep Sci, 2007, 30:2647
13. [13]
  [13] Delaunay-Bertoncini N, Hennion M C. J Pharm Biomed Anal, 2004, 34:717
14. [14]
  [14] Senyuva H Z, Gilbert J. J Chromatogr B, 2010, 878:115
15. [15]
  [15] Wang H, Zhao L, Yang H M, et al. Chinese Journal of Chromatography, 2015, 33(9):995 王浩, 赵丽, 杨红梅, 等. 色谱, 2015, 33(9):995
16. [16]
  [16] Huang H, Chen J H, Feng N, et al. Science and Technology of Food Industry, 2013, 33(4):378 黄华, 陈君慧, 冯楠, 等. 食品工业科技, 2013, 33(4):378
17. [17]
  [17] KungT A, Tsai C A, Ku B C, et al. Food Chem, 2015, 175:189
18. [18]
  [18] He J H, Qiu J R, He D C, et al. Environmental Chemistry, 2012, 31(9):1436 何金华, 丘锦荣, 贺德春, 等. 环境化学, 2012, 31(9):1436
19. [19]
  [19] Li X D, Xian Q M, Liu H L, et al. Chinese Journal of Analytical Chemistry, 2010, 38(3):429 李学德, 鲜啟明, 刘红玲, 等. 分析化学, 2010, 38(3):429
20. [20]
  [20] Liu J H, Sun Z Z, Huang X L, et al. Chinese Journal of Chromatography, 2015, 33(4):434 刘菁华, 孙振中, 黄雪玲, 等. 色谱, 2015, 33(4):434
21. [21]
  [21] Wu C Q, Lei J M, Li Y L, et al. Chinese Journal of Chromatography, 2014, 32(12):1362 吴翠琴, 雷金妹, 李韵灵, 等. 色谱, 2014, 32(12):1362
22. [22]
  [22] Deng Y H, Li L, Zhang H Q, et al. Journal of Huazhong Normal University, 2014, 48(1):53 邓樱花, 李林, 张洪权, 等. 华中师范大学学报, 2014, 48(1):53
23. [23]
  [23] Yoshitake M, Nohta H, Ogata S, et al. J Chromatogr B, 2007, 858:307
24. [24]
  [24] Arroyo-Manzanares N, Gámiz-Gracia L, García-Campaña A M. Food Chem, 2014, 143:459
25. [25]
  [25] Kang J, Fan C L, Chang Q Y, et al. Anal Methods, 2014, 6:6285
26. [26]
  [26] Xiong S X, Han H W, Zhao R, et al. Chemical Journal of Chinese Universities, 2000, 21(8):1191 熊少祥, 韩慧婉, 赵睿, 等. 高等学校化学学报, 2000, 21(8):1191
27. [27]
  [27] Amankwa L N, Scholl J, Kuhr W G. Anal Chem, 1990, 62:2189
28. [28]
  [28] O'shea T J, Weber P L, Bammel B P, et al. J Chromatogr, 1992, 608(1/2):189
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