Citation: LI Liqun, FAN Jun, ZHANG Jing, CHEN Xiaodong, WANG Tai, HE Jianfeng, ZHANG Weiguang. Enantioseparation behavior of chiral stationary phases AD, AS and OD[J]. Chinese Journal of Chromatography, ;2016, 34(1): 108-112. doi: 10.3724/SP.J.1123.2015.10035 shu

Enantioseparation behavior of chiral stationary phases AD, AS and OD

LI Liqun ¹ ,
FAN Jun ^1,, ,
ZHANG Jing ¹ ,
CHEN Xiaodong ² ,
WANG Tai ² ,
HE Jianfeng ¹ ,
ZHANG Weiguang ^1,2,,

1.
1. School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China;
2.
2. Guangzhou Research & Creativity Biotechnology Co. Ltd., Guangzhou 510663, China

Corresponding author: FAN Jun, ZHANG Weiguang,
Received Date: 24 October 2015

Fund Project: 国家自然科学基金项目(21171059,21571070) (21171059,21571070)科技部中小型企业技术创新基金项目(13C26214404534) (13C26214404534)广东省科技计划项目(2014A010101145,2012B010900043) (2014A010101145,2012B010900043)广州市科技计划项目(2013J4400027,201508020093). (2013J4400027,201508020093)

Over the past decades, HPLC enantioseparation with chiral stationary phases (CSPs) has been widely applied in chiral analysis and preparation of new pharmaceuticals, pesticides, food, etc. Herein, enantioseparation of 20 chiral compounds have been carried out on three polysaccharide-based CSPs (EnantioPak AD, AS and OD) with normal phases by HPLC, separately. The influences of skeletal structure and the kinds of derivative groups on separation behaviors of these CSPs have been studied in detail. As results indicated, except for compound 13, the other compounds were baseline separated on EnantioPak AD, with most of resolution over 2.0; in addition, better separation for acidic or basic compounds was achieved through adding acidic/basic additives into the mobile phase of hexane-alcohol. For four aromatic alcohols (compounds 13-16), their retention in the EnantioPak AD column showed a weakening tendency with increase of carbon number in side chain group, and the reverse trend of their resolution was observed. Furthermore, EnantioPak AD showed much better separation performance for eight compounds (13-20) than the others. In short, these results have provided some references for further investigation of separation behavior and applications of polysaccharide-based CSPs.
- polysaccharide-based chiral stationary phases,
- high performance liquid chromatography (HPLC),
- chiral recognition,
- separation mechanism
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