Citation: XU Lili, CHEN Geyu, ZHANG Teng, ZHANG Zhongqi, HAN Guang, LI Qian, ZHAO Jinli, LIU Jianli. Preparation and properties of new nickel ion affinity chromatography medium[J]. Chinese Journal of Chromatography, ;2016, 34(3): 232-239. doi: 10.3724/SP.J.1123.2015.10030 shu

Preparation and properties of new nickel ion affinity chromatography medium

XU Lili ¹ ,
CHEN Geyu ¹ ,
ZHANG Teng ¹ ,
ZHANG Zhongqi ² ,
HAN Guang ² ,
LI Qian ² ,
ZHAO Jinli ² ,
LIU Jianli ^1,,

1.
1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an 710069, China;
2.
2. Active Protein & Polypeptide Engineering Center of Xi'an Hua Ao Li Kang, Xi'an 710054, China

Corresponding author: LIU Jianli,
Received Date: 20 October 2015

Fund Project: 国家自然科学基金项目(30070905,20872118) (30070905,20872118)陕西省重点实验室基金项目(2010JS097) (2010JS097)陕西省教育厅基金项目(08jk477). (08jk477)

With the chloromethylated crosslinking polystyrene microspheres (chlorine ball) as the matrices, N,N-dimethylformamide (DMF) as the solvent, activated by N,N-diisopropylethylamine (DIEA), the synthesized Lys(Boc)-OEt and ethyl chloroacetate were coupled to the matrices. After hydrolysis, the carboxyl group was released and chelated with Ni²⁺, and a new nickel ion affinity chromatographic medium was prepared. The affinity medium was applied to purify the His-Lys-Tyr and Phe-His-Thr tri-peptides. The results showed that the peptides containing histidine were retained on the affinity medium and separated with other substances.
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