Citation: LI Juan, QIAO Qingdong, ZHUANG Jingxin, WANG Cuiping. Simultaneous determination of the residues of four β₂-agonists in animal foods by modified high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(2): 170-175. doi: 10.3724/SP.J.1123.2015.08033 shu

Simultaneous determination of the residues of four β₂-agonists in animal foods by modified high performance liquid chromatography-tandem mass spectrometry

1.
Beijing Changping District Center for Disease Control and Prevention, Beijing 102200, China

Corresponding author: LI Juan,
Received Date: 27 August 2015

Fund Project: 中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室开放基金项目(KF2014-04). (KF2014-04)

An analytical method was developed to simultaneously determine the residues of four β₂-agonists in animal foods by high performance liquid chromatography/electrospray-tandem mass spectrometry (HPLC-MS/MS). Samples were extracted with 5% (mass fraction) trichloracetic acid, and then cleaned up by two solid phase extraction cartridges (HLB and ProElut PXC). Quantification of the four β₂-agonists was achieved by HPLC-MS/MS in multiple reaction monitoring (MRM) using internal standard method. The limits of detection (S/N=3) of clenbuterol, salbutamol, ractopamine and terbutaline were 0.05, 0.05, 0.05 and 0.2 μ g/kg, and the limits of quantification (S/N=10) were 0.25, 0.25, 0.1 and 0.5 μ g/kg, respectively. The average recoveries of the four β₂-agonists spiked in blank samples at the spiked levels of 2.5, 5 and 10 μ g/kg were 90.3%-120.5% with the relative standard deviation (RSD) range of 1.60%-9.33%. The method is reliable, sensitive, good recovery and repeatability. It is suitable for the determination of the residues of the four β₂-agonists in animal foods.
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- solid phase extraction,
- acidolysis extraction,
- β₂-agonists,
- animal foods
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沈阳化工大学材料科学与工程学院沈阳 110142

Simultaneous determination of the residues of four β2-agonists in animal foods by modified high performance liquid chromatography-tandem mass spectrometry

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通讯作者: 陈斌, bchen63@163.com

Simultaneous determination of the residues of four β₂-agonists in animal foods by modified high performance liquid chromatography-tandem mass spectrometry