Citation: LIN Tao, FAN Jianlin, LIU Xingyong, CHEN Xinglian, LI Yangang, LIU Hongcheng. Determination of amantadine and rimantadine residues in egg and chicken samples by dispersive solid phase extraction purification-ultra high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(11): 1169-1174. doi: 10.3724/SP.J.1123.2015.08010 shu

Determination of amantadine and rimantadine residues in egg and chicken samples by dispersive solid phase extraction purification-ultra high performance liquid chromatography-tandem mass spectrometry

LIN Tao ^1,2 ,
FAN Jianlin ¹ ,
LIU Xingyong ¹ ,
CHEN Xinglian ¹ ,
LI Yangang ¹ ,
LIU Hongcheng ^1,2,,

1.
1. Institute of Quality Standards & Testing Technique, Yunnan Academy of Agricultural Science, Kunming 650223, China;

Corresponding author: LIU Hongcheng,
Received Date: 9 August 2015

Fund Project: 云南省社会发展科技计划项目(2013RA012) (2013RA012)云南省应用基础研究计划青年项目(2013FD064) (2013FD064)云南省科技创新平台建设计划项目(2014DA001). (2014DA001)

A method was developed for the determination of residual amantadine and rimantadine in eggs and chickens by dispersive solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry. Egg and chicken samples were extracted with ammonia water-acetonitrile (2 : 98, v/v). The extraction solution was dried to 1 mL under nitrogen, and then purified by dispersive solid phase extraction method with C18 and NH₂ sorbents. After purification, the extraction solution was filtered through a filter. The target compounds were analyzed by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) on a ZORBAX C18 column using a mixture of 1 mmol/L ammonium acetate solution (containing 0.1% (v/v) formic acid) and methanol as mobile phases with gradient elution. The mass spectrometer was operated under multiple reaction monitoring (MRM) mode in positive mode. The good linearities were obtained for amantadine and rimantadine at a concentration range of 0.15-10.0 μ g/L. The limits of detection for amantadine and rimantadine were all 0.05 μ g/kg, and the limits of quantification were 0.20 μ g/kg. The recoveries of amantadine and rimantadine in eggs and chickens at three spiked levels (0.2, 1.0 and 2.0 μ g/kg) were in the range of 89%-108% with the relative standard deviations of 5.0%-8.6%. The results demonstrated that the method is suitable for the determination of amantadine and rimantadine in eggs and chickens.
- dispersive solid phase extraction,
- ultra high performance liquid chromatography (UHPLC),
- tandem mass spectrometry (MS/MS),
- amantadine,
- rimantadine,
- eggs,
- chickens
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