Citation: YAN Jingyu, GUO Zhimou, DING Junjie, SHEN Aijin, WANG Jixia, JIN Gaowa, LIANG Xinmiao. Analysis of vancomycin and its related impurities by hydrophilic interaction liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(9): 951-956. doi: 10.3724/SP.J.1123.2015.05032 shu

Analysis of vancomycin and its related impurities by hydrophilic interaction liquid chromatography

1.
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Corresponding author: YAN Jingyu,
Received Date: 26 May 2015

Fund Project: 国家自然科学基金项目(21207130). (21207130)

The current reversed-phase liquid chromatography as the main analytical method for vancomycin is inappropriate due to its poor polarity selectivity and mass spectrometry compatibility. Hydrophilic interaction chromatography (HILIC) method has been verified to provide the glycopeptide substances with good retention and polarity selectivity, therefore, an HILIC method was developed for the analysis of vancomycin and its related impurities including norvancomycin, desvancosaminyl vancomycin, dedichloro vancomycin and crystalline degradation product (CDP-1). In this work, a systematic evaluation of retention behavior of vancomycin and its related impurities in hydrophilic interaction chromatography was performed and the influences of stationary phase, acetonitrile content, buffer salt and pH of mobile phase were studied. According to the results, Click XIon column was selected as stationary phase and ammonium formate was additive in the mobile phase. Vancomycin and its related impurities were totally separated under the optimization conditions. It is concluded that hydrophilic interaction chromatography may be a new strategy for the separation of vancomycin and its structural analogues.
- hydrophilic interaction chromatography (HILIC),
- vancomycin,
- glycopeptides antibiotics,
- impurities analysis
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沈阳化工大学材料科学与工程学院沈阳 110142