Citation: JIANG Ge, SHEN Aijin, GUO Zhimou, LI Xiuling, LIANG Xinmiao. Evaluation of retention behavior of carbohydrate compounds on the zwitterionic hydrophilic interaction liquid chromatographic column[J]. Chinese Journal of Chromatography, ;2015, 33(9): 929-933. doi: 10.3724/SP.J.1123.2015.05011 shu

Evaluation of retention behavior of carbohydrate compounds on the zwitterionic hydrophilic interaction liquid chromatographic column

1.
Dalian Institute of Chemistry and Physics, Key Laboratory of Separation Science for Analytical Chemistry, Chinese Academy of Sciences, Dalian 116023, China

Corresponding author: LI Xiuling,
Received Date: 11 May 2015

Fund Project: 国家自然科学基金项目(21475129). (21475129)

Because of the strong polarity of carbohydrate compounds which have weak retention in reverse-phase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC) is suitable for the separation and analysis of the carbohydrate compounds. In this study, nine carbohydrate compounds were selected as the test probes to systematically evaluate the retention behavior of the carbohydrate compounds on a Click TE-Cys column with zwitterionic stationary phase. And the effects of the ratio of organic phase and salt concentration in mobile phase on the retention behavior were investigated as well. The experiments proved that the nine carbohydrate compounds could be eluted in the order of their polarities from the Click TE-Cys column. With the increase of organic phase ratio, the retention of carbohydrate compounds was enhanced. As the increase of salt concentration, the retention of the carbohydrate compounds increased except sialic acid. Using displacement-adsorption liquid phase interaction model to simulate the retention behavior of the carbohydrate compounds under HILIC, the retention behavior of HILIC was described by the retention equation of ln k=a+bln C_B+cC_B, and the retention values of HILIC were analyzed by multiple linear regression. The results demonstrated that the retention behavior of carbohydrate compounds on the Click TE-Cys was in accordance with the retention regularity of HILIC.
- zwitterionic stationary phase,
- hydrophilic interaction liquid chromatography (HILIC),
- Click TE-Cys column,
- retention behavior,
- oligosaccharide compounds
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沈阳化工大学材料科学与工程学院沈阳 110142