Citation: CHU Ganghui, YIN Xuebo, . Determination of rutin, quercetin and kaempferol in althaea rosea (L) Gavan for Uyghur medicine by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(12): 1269-1273. doi: 10.3724/SP.J.1123.2015.05008 shu

Determination of rutin, quercetin and kaempferol in althaea rosea (L) Gavan for Uyghur medicine by high performance liquid chromatography

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CHU Ganghui ^1,2 ,
YIN Xuebo ^1,2 ,
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1.
1. College of Chemistry and Environmental Sciences, Kashgar University, Kashgar 844006, China;

Corresponding author: ,
Received Date: 8 May 2015

Fund Project: 新疆维吾尔自治区高校科研计划重点课题项目(XJEDU2011147). (XJEDU2011147)

Uyghur medicine is one important part of the national medicine system. Uyghur medicine modernization, namely the study of effective components with modern technologies, is the only way for the scientification, standardization, and industrialization of Uyghur medicine. Here we developed a selective extraction method for rutin, quercetin and kaempferol in Althaea rosea (L) Gavan. The three active species were determined by high performance liquid chromatography (HPLC) with HC-C18 column (250 mm×4.6 mm, 5 μm) and the mobile phase of CH₃OH-0.4% H₃PO₄(50:50, v/v). Rutin, quercetin and kaempferol were baseline separated with each other and the interference species with flow rate of 1.0 mL/min and column temperature of 30 ℃. Under the optimal conditions, linear correlation were obtained in the mass concentration range of 12.5-150 μg/mL (r=0.9998) for rutin, 12.5-125 μg/mL (r=0.9999) for quercetin, and 12.5-125 μg/mL (r=0.9988) for kaempferol. The recoveries (n=5) of rutin, quercetin and kaempferol were 100.25% (RSD=1.1%), 97.60% (RSD=0.47%) and 97.75% (RSD=0.71%), respectively. The method can be used to determine the contents of rutin, quercetin and kaempferol in Althaea rosea (L) Gavan and provide the guidance for the analysis of the flavonoids in other Uyghur medicines.
- high performance liquid chromatography (HPLC),
- rutin,
- quercetin,
- kaempferol,
- Althaea rosea (L) Gavan
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