Citation: CHU Ganghui, YIN Xuebo, . Determination of rutin, quercetin and kaempferol in althaea rosea (L) Gavan for Uyghur medicine by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(12): 1269-1273. doi: 10.3724/SP.J.1123.2015.05008 shu

Determination of rutin, quercetin and kaempferol in althaea rosea (L) Gavan for Uyghur medicine by high performance liquid chromatography

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CHU Ganghui ^1,2 ,
YIN Xuebo ^1,2 ,
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1.
1. College of Chemistry and Environmental Sciences, Kashgar University, Kashgar 844006, China;

Corresponding author: ,
Received Date: 8 May 2015

Fund Project: 新疆维吾尔自治区高校科研计划重点课题项目(XJEDU2011147). (XJEDU2011147)

Uyghur medicine is one important part of the national medicine system. Uyghur medicine modernization, namely the study of effective components with modern technologies, is the only way for the scientification, standardization, and industrialization of Uyghur medicine. Here we developed a selective extraction method for rutin, quercetin and kaempferol in Althaea rosea (L) Gavan. The three active species were determined by high performance liquid chromatography (HPLC) with HC-C18 column (250 mm×4.6 mm, 5 μm) and the mobile phase of CH₃OH-0.4% H₃PO₄(50:50, v/v). Rutin, quercetin and kaempferol were baseline separated with each other and the interference species with flow rate of 1.0 mL/min and column temperature of 30 ℃. Under the optimal conditions, linear correlation were obtained in the mass concentration range of 12.5-150 μg/mL (r=0.9998) for rutin, 12.5-125 μg/mL (r=0.9999) for quercetin, and 12.5-125 μg/mL (r=0.9988) for kaempferol. The recoveries (n=5) of rutin, quercetin and kaempferol were 100.25% (RSD=1.1%), 97.60% (RSD=0.47%) and 97.75% (RSD=0.71%), respectively. The method can be used to determine the contents of rutin, quercetin and kaempferol in Althaea rosea (L) Gavan and provide the guidance for the analysis of the flavonoids in other Uyghur medicines.
- high performance liquid chromatography (HPLC),
- rutin,
- quercetin,
- kaempferol,
- Althaea rosea (L) Gavan
1. [1]
  [1] Compiling Group of Glossary of Chinese Traditional and Herbal Drugs. Glossary of Chinese Traditional and Herbal Drugs. 2nd ed. Beijing: People's Medical Publishing House (PMPH)(《全国中草药汇编》编写组. 全国中草药汇编. 第2版. 北京: 人民卫生出版社), 1996: 648
2. [2]
  [2] Alpna J. Advances in Plant Sciences, 2013, 26(1): 153
3. [3]
  [3] Eskandari M, Samavati V. Int J Biol Macromol, 2015, 72: 347
4. [4]
  [4] Yinayetula, Hailiqiemu. Uighur Medicinal Materials. Urumqi: Xinjiang Science and Technology Press (伊那耶吐拉, 海力且木. 维吾尔常用药材. 乌鲁木齐: 新疆科技卫生出版社), 1991: 156
5. [5]
  [5] Jiangsu New Medical College. Dictionary of Chinese Medicinal Herb. Shanghai: Shanghai Science and Technology Press (江苏新医学院. 中药大辞典. 上海: 上海科学技术出版社), 1986: 2488
6. [6]
  [6] Feng Y L, Xu L Z, Yang S L, et al. Chinese Traditional and Herbal Drugs (冯育林, 徐丽珍, 杨世林, 等. 中草药), 2005, 36(11): 1610
7. [7]
  [7] Feng Y L, Li Y Q, Xu L Z, et al. Chinese Traditional and Herbal Drugs (冯育林, 李云秋, 徐丽珍, 等. 中草药), 2006, 37(11): 1622
8. [8]
  [8] Zhang W, Chen Q, Liu L L, et al. Journal of Shenyang Pharmaceutical University (张祎, 陈秋, 刘丽丽, 等. 沈阳药科大学学报), 2013, 30(5): 335
9. [9]
  [9] Mian Q H, Zheng D C, Sun L. Journal of Xinjiang Medical University (勉强辉, 郑大成, 孙莲. 新疆医科大学学报), 2014, 37(2): 160
10. [10]
  [10] Shang Z C, Wang B C, Zhang W W, et al. China Pharmacy (尚志春, 王宝昌, 张微微, 等. 中国药房), 2013, 24(27): 2526
11. [11]
  [11] Xu J Y, Li S. Chinese Journal of Experimental Traditional Medical Formulae (许继艳, 李硕. 中国实验方剂学杂志), 2014, 20(12): 69
12. [12]
  [12] Zhang J C, Wei J, Zhong H M, et al. Chinese Journal of Chromatography (张敬彩, 魏杰, 钟虹敏, 等. 色谱), 2013, 31(1): 79
13. [13]
  [13] Mao X Q, Bian H T, Qu B C. Chinese Journal of Chromatography (毛希琴, 边海涛, 曲宝成. 色谱), 2013, 31(8): 775
14. [14]
  [14] Qi L, Zhang J, Zhang Z Q. Chinese Journal of Chromatography (亓亮, 张婧, 张志琪. 色谱), 2013, 31(3): 249
15. [15]
  [15] Zhao F, Gao G H, Na H Q, et al. Chinese Journal of Chromatography (赵飞, 高广慧, 那海秋, 等. 色谱), 2013, 31(5): 490
16. [16]
  [16] National Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China, Part 1. Beijing: Chemical Industry Press (国家药典委员会编. 中华人民共和国药典, 一部. 北京: 中国医药科技出版社), 2010: 1081
17. [17]
  [17] Zhou R, Cao Z Y, Mou R X, et al. Chinese Journal of Chromatography (周蓉, 曹赵云, 牟仁祥, 等. 色谱), 2015, 33(1): 35
18. [18]
  [18] Chen H, Huang Y, Zhai J L. Chinese Journal of Pharmaceutical Analysis (陈晖, 黄燕, 瞿继兰. 药物分析杂志), 2012, 32(9): 1574
19. [19]
  [19] Zhou N, Yang Q, Yang M, et al. Chinese Journal of Pharmaceutical Analysis (周浓, 杨勤, 杨敏, 等. 药物分析杂志), 2013, 33(2): 225
20. [20]
  [20] Zhang Y, Xue K P, Zhou A, et al. Chinese Journal of Chromatography (张勇, 薛昆鹏, 周安, 等. 色谱), 2014, 32(1): 47
21. [21]
  [21] Li Z C, Chen L Y, Guo S H. Journal of Analytical Science (林珠灿, 陈丽云, 郭素华. 分析科学学报), 2013, 29(6): 819
22. [22]
  [22] Huang W, Gao J, Cheng W M, et al. Asian J Chem, 2014, 26(11): 3134
23. [23]
  [23] Xie Z S, Lam S C, Wu J W, et al. Anal Methods, 2014, 6 (12): 4328
24. [24]
  [24] Parvu M, Toiu A, Vlase L, et al. Nat Prod Res, 2010, 24(14): 1318
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