Citation: HAO Weiqiang, WANG Leijun, WU Shun, YUE Bangyi, CHEN Qiang, ZHANG Peipei. Analysis of alkaline CuO degradation products of acid detergent fiber from tobacco leaves by using liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(7): 777-782. doi: 10.3724/SP.J.1123.2015.01043 shu

Analysis of alkaline CuO degradation products of acid detergent fiber from tobacco leaves by using liquid chromatography

HAO Weiqiang ^1,2,, ,
WANG Leijun ¹ ,
WU Shun ² ,
YUE Bangyi ² ,
CHEN Qiang ² ,
ZHANG Peipei ²

1.
1. Key Laboratory of Cigarette Smoke Research, Shanghai Tobacco Group Co., Ltd., China National Tobacco Corporation (CNTC), Shanghai 200082, China;

Corresponding author: HAO Weiqiang,
Received Date: 29 January 2015

Fund Project: 烟草行业卷烟烟气重点实验室开放研究基金项目(SZBCW201300555) (SZBCW201300555)江苏省自然科学基金项目(BK20141163). (BK20141163)

The acid detergent fiber (ADF) from tobacco leaves was obtained by treating the sample with petroleum ether-ethanol (6 : 4, v/v), 30 g/L sodium dodecylsulfate and 0.5 mol/L sulphuric acid containing 20 g/L hexadecyl trimethyl ammonium bromide successively. The ADF was degraded by the alkaline CuO oxidation procedure. In this work, six samples of ADF degradation products from tobacco leaves were prepared. The samples were analyzed by using gradient liquid chromatography (LC) where an Ultimate XB C18 column was used as stationary phase, with a mixture of methanol and water as mobile phase, at a column temperature of 35 ℃ and a flow rate of 0.8 mL/min. Dual wavelengths of 280 nm and 320 nm were chosen for the detection. It was found that there were four characteristic peaks for the ADF degradation products. By taking these peaks as research object, the optimum time for the degradation was found to be 5 h and the sample solution could be kept stable within 7 days. The established method may provide a new approach for the studies of the differences between lignin composition in different tobacco leaves and the relationship between lignin content and the smoking quality of tobacco leaves.
- liquid chromatography (LC),
- acid detergent fiber (ADF),
- alkaline CuO degradation,
- lignin,
- tobacco leaves
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