Citation: WANG Hui, XUE Yun, LU Yangfang, GU Xue, WANG Yan, YAN Chao. Preparation and evaluation of glutathione modified SiO₂@Au as stationary phase in capillary liquid chromatography and pressurized capillary electrochromatography[J]. Chinese Journal of Chromatography, ;2015, 33(3): 215-220. doi: 10.3724/SP.J.1123.2014.12002 shu

Preparation and evaluation of glutathione modified SiO₂@Au as stationary phase in capillary liquid chromatography and pressurized capillary electrochromatography

1.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: WANG Yan, YAN Chao,
Received Date: 1 December 2014
Available Online: 4 January 2015
Fund Project: 国家自然科学基金项目(21175092,21105064) (21175092,21105064)国家重大科学仪器设备开发专项(2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210) (2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210)上海市自然科学基金项目(12ZR1413600). (12ZR1413600)

In order to improve the contents of -NH₂ group on the surface of SiO₂, polyethylenimine (PEI) was introduced on the surface of SiO₂ through Schiff Base reaction. Then self-assembled monolayer electroless plating (SAM-electroless plating) method was used to prepare SiO₂@Au core-shell particles. During this process, two factors were discussed. They were the reaction pH in the SiO₂@Au seed forming phase and the reaction volume of formaldehyde in the SiO₂@Au seed growth phase. The scanning electronic microscopy (SEM) showed the coverage of Au was high and the dispersity was uniform after the optimizations. As we know, Au can coordinate with -SH group easily, so glutathione (GSH) can be bound on the surface of Au. In this study, GSH was bound on the surface of Au through the optimization of the reaction pH. The Fourier transform infrared spectrum showed that the GSH was bound on the surface of Au successfully at pH 3. At last, this packing material was packed into capillary column by slurry packing method to prepare SiO₂@Au-GSH capillary columns, and the capillary liquid chromatography (cLC) mode was used to certify the hydrophilic property of this kind of column. In addition, this kind of column was certified to have a good separation ability of polar compounds in pressurized capillary electrochromatography (pCEC) mode.
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沈阳化工大学材料科学与工程学院沈阳 110142

Preparation and evaluation of glutathione modified SiO2@Au as stationary phase in capillary liquid chromatography and pressurized capillary electrochromatography

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通讯作者: 陈斌, bchen63@163.com

Preparation and evaluation of glutathione modified SiO₂@Au as stationary phase in capillary liquid chromatography and pressurized capillary electrochromatography